Steele J G, Dalton B A, Johnson G, Underwood P A
CSIRO Division of Biomolecular Engineering, Sydney Laboratories, North Ryde, NSW, Australia.
Biomaterials. 1995 Sep;16(14):1057-67. doi: 10.1016/0142-9612(95)98901-p.
The two cell culture substrata, tissue culture polystyrene (TCPS) and Primaria, were compared in order to determine whether a nitrogen-containing surface such as Primaria attracts cells by a different mechanism to an oxygen-containing surface (TCPS). The amounts of vitronectin (Vn) and fibronectin (Fn) which adsorb from the fetal bovine serum (FBS) component of the culture medium onto Primaria and TCPS were determined. Primaria adsorbed two- to threefold more Fn than TCPS, but adsorbed similar amounts of Vn from medium containing FBS. The Fn and Vn binding sites on Primaria were distinct, as adsorption was non-competitive between these two proteins. The amounts of Fn and Vn that adsorbed onto the two surfaces were compared to the concentration dependence of the cell attachment activities of Fn and Vn. Whereas the amounts of Fn which adsorbed onto TCPS were suboptimal for cell attachment, Primaria adsorbed an Fn surface density that was supraoptimal for attachment of human vein endothelial cells and BHK-21 fibroblasts. We conclude that Primaria differs from TCPS in that both Fn and Vn mediate initial cell attachment to Primaria when the culture medium contains FBS, whereas cell attachment to TCPS is dependent upon Vn.
为了确定含氮表面（如Primaria）吸引细胞的机制是否与含氧表面（组织培养聚苯乙烯，TCPS）不同，对两种细胞培养基质，即组织培养聚苯乙烯（TCPS）和Primaria进行了比较。测定了从培养基的胎牛血清（FBS）成分吸附到Primaria和TCPS上的玻连蛋白（Vn）和纤连蛋白（Fn）的量。Primaria吸附的Fn比TCPS多两到三倍，但从含FBS的培养基中吸附的Vn量相似。Primaria上的Fn和Vn结合位点是不同的，因为这两种蛋白质之间的吸附是非竞争性的。将吸附在这两种表面上的Fn和Vn的量与Fn和Vn的细胞附着活性的浓度依赖性进行了比较。虽然吸附在TCPS上的Fn量对于细胞附着来说是次优的，但Primaria吸附的Fn表面密度对于人静脉内皮细胞和BHK - 21成纤维细胞的附着来说是超优的。我们得出结论，Primaria与TCPS的不同之处在于，当培养基含有FBS时，Fn和Vn都介导细胞最初附着到Primaria上，而细胞附着到TCPS则依赖于Vn。
