Rossi S, Bonferoni M C, Lippoli G, Bertoni M, Ferrari F, Caramella C, Conte U
Department of Pharmaceutical chemistry, University of Pavia, Italy.
Biomaterials. 1995 Sep;16(14):1073-9. doi: 10.1016/0142-9612(95)98903-r.
There are numerous in vitro methods with which to investigate the mucoadhesive properties of polymers. One recent method is based on the measurement of rheological interactions between polymer and mucin, which implies the use of mucins isolated from the mucous tissue. The extraction and purification of glycoprotein fraction, which is responsible for rheological interaction, can modify the native structure of mucin or spoil it with exogenous substances. Therefore the particulars of the mucin employed (origin, purification grade, the effect of further treatments such as freezing or freeze-drying) are likely to be critical for the interaction. The aim of this work was to compare some commercial mucins of differing origin and grade of purification for their rheological interaction with well-known mucoadhesive polymers (polyacrylic acid and sodium carboxymethylcellulose). For polyacrylic acid, which is sensitive to ions, we found rheological interaction to be strongly influenced by mucin type. The removal of ions, with dialysis, improved the interaction. For sodium carboxymethylcellulose, which is less sensitive to ions, rheological interaction proved to be less dependent on mucin type and improved upon glycoprotein solubilization.
有许多体外方法可用于研究聚合物的粘膜粘附特性。最近的一种方法是基于测量聚合物与粘蛋白之间的流变相互作用，这意味着要使用从粘液组织中分离出来的粘蛋白。负责流变相互作用的糖蛋白部分的提取和纯化可能会改变粘蛋白的天然结构或被外源性物质破坏。因此，所使用粘蛋白的具体情况（来源、纯化等级、进一步处理如冷冻或冻干的影响）可能对相互作用至关重要。这项工作的目的是比较一些不同来源和纯化等级的市售粘蛋白与知名粘膜粘附聚合物（聚丙烯酸和羧甲基纤维素钠）的流变相互作用。对于对离子敏感的聚丙烯酸，我们发现流变相互作用受粘蛋白类型的强烈影响。通过透析去除离子可改善相互作用。对于对离子不太敏感的羧甲基纤维素钠，流变相互作用被证明较少依赖于粘蛋白类型，并且在糖蛋白溶解后得到改善。
