Fuongfuchat A, Jamieson A M, Blackwell J, Gerken T A
Department of Macromolecular Science, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Carbohydr Res. 1996 Apr 18;284(1):85-99. doi: 10.1016/0008-6215(95)00396-7.
The associative interaction of purified ovine and porcine submaxillary mucins (OSM and PSM) with sodium alginate was evaluated by comparing the rheological properties of mixtures against those of pure alginate and mucin in dilute, semi-dilute, and concentrated solutions. These systems were investigated as models for the interaction of mucin with the extracellular alginate produced by Pseudomonas aeruginosa. In dilute solution, evidence for such interaction cannot be obtained because aggregate species exist both in the OSM-alginate mixtures as well as in pure OSM. However, in the semi-dilute regime, mixtures containing a higher proportion of mucin show systematically higher viscosities than those predicted by simple additivity. In concentrated solutions containing higher proportions of mucin, an enhanced elastic response is observed. These results demonstrate a substantial binding interaction of mucins with alginate. This property is not observed in mixtures containing a high proportion of alginate, suggesting that mucins possess relatively low numbers of interacting sites. Introduction of 3 mM Ca2+ ions to all mucin-alginate mixtures enhances the elasticity due to gelation of alginate. Finally, comparison of the rheological properties of PSM-alginate mixtures with those of PSM-polyacrylate mixtures indicates that the binding strength of alginate to mucin is significantly weaker than that of polyacrylate.
通过比较纯化的绵羊和猪下颌下粘蛋白（OSM和PSM）与海藻酸钠混合物在稀溶液、半稀溶液和浓溶液中的流变学性质，评估了它们之间的缔合相互作用。这些体系被作为粘蛋白与铜绿假单胞菌产生的细胞外海藻酸钠相互作用的模型进行研究。在稀溶液中，由于OSM-海藻酸钠混合物以及纯OSM中都存在聚集体，所以无法获得这种相互作用的证据。然而，在半稀溶液体系中，含有较高比例粘蛋白的混合物表现出比简单加和预测值更高的粘度。在含有较高比例粘蛋白的浓溶液中，观察到弹性响应增强。这些结果表明粘蛋白与海藻酸钠之间存在显著的结合相互作用。在含有高比例海藻酸钠的混合物中未观察到这种性质，这表明粘蛋白具有相对较少的相互作用位点。向所有粘蛋白-海藻酸钠混合物中加入3 mM Ca2+离子会由于海藻酸钠的凝胶化而增强弹性。最后，PSM-海藻酸钠混合物与PSM-聚丙烯酸酯混合物流变学性质的比较表明，海藻酸钠与粘蛋白的结合强度明显弱于聚丙烯酸酯。
