Kwak Y S, Kobayashi T, Akiba T, Horikoshi K, Kim Y B
Korea Ginseng and Tobacco Research Institute, Taejon.
Biosci Biotechnol Biochem. 1995 Sep;59(9):1666-9. doi: 10.1271/bbb.59.1666.
A hyperthermophilic archaebacterium was isolated from a deep-sea black smoker chimney (depth, 760 m) at the Minami-ensei Knoll (28 degrees 23'N, 127 degrees 38'E). The strain, designated DT1331, was a coccoid shaped bacterium about 0.5 to 1.0 microns in diameter. The cells were surrounded by a cell envelope. The temperature for growth was between 55 degrees C and 93 degrees C with an optimum 80 degrees C. The growth occurred from pH 4.5 to 8.5 and the optimum pH was 6.0. DT1331 required 1% to 5% NaCl for growth and cell lysis was observed below 1% NaCl concentration. The strain was an anaerobic chemoorganotroph requiring elemental sulfur obligately. Organic substrates used included tryptone, peptone, soytone, casein, gelatin, and yeast extract. Under the optimal conditions, DT1331 had a generation time of 50 min and could reach densities of about 1.5 x 10(8) cells/ml. DT1331 was resistant to ampicillin, chloramphenicol, erythromycin, kanamycin, streptomycin, and tetracycline, which was one of the common characteristics of archaebacteria. The G+C content of DT1331 was 52.3 mol%. Analysis of the 16S rRNA gene by restriction enzymes coincided with those of Thermococcus celer, indicating that this strain belonged to the genus Thermococcus.
从南伊豆海山（北纬28度23分，东经127度38分）水深760米的深海热液喷口烟囱中分离出一株嗜热古细菌。该菌株命名为DT1331，是一种直径约0.5至1.0微米的球状细菌。细胞被细胞膜包围。生长温度在55℃至93℃之间，最适温度为80℃。生长pH范围为4.5至8.5，最适pH为6.0。DT1331生长需要1%至5%的NaCl，在NaCl浓度低于1%时观察到细胞裂解。该菌株是一种厌氧化学有机营养型细菌，专一需要单质硫。使用的有机底物包括胰蛋白胨、蛋白胨、大豆蛋白胨、酪蛋白、明胶和酵母提取物。在最佳条件下，DT1331的代时为50分钟，细胞密度可达约1.5×10⁸个/毫升。DT1331对氨苄青霉素、氯霉素、红霉素、卡那霉素、链霉素和四环素具有抗性，这是古细菌的共同特征之一。DT1331的G+C含量为52.3摩尔%。用限制性内切酶对16S rRNA基因进行分析，结果与嗜热栖热球菌一致，表明该菌株属于嗜热栖热球菌属。
