来自酱油曲霉菌米曲霉KBN616的硝酸还原酶基因。

The nitrate reductase gene from a shoyu koji mold, Aspergillus oryzae KBN616.

作者信息

Kitamoto N, Kimura T, Kito Y, Ohmiya K, Tsukagoshi N

机构信息

Food Research Institute, Aichi Prefectural Government, Nagoya, Japan.

出版信息

Biosci Biotechnol Biochem. 1995 Sep;59(9):1795-7. doi: 10.1271/bbb.59.1795.

DOI:10.1271/bbb.59.1795

PMID:8520125

Abstract

A niaD gene encoding nitrate reductase was isolated from Aspergillus oryzae KBN616 and sequenced. The structural gene comprises 2973 bp and 868 amino acids, which showed a high degree of similarity to nitrate reductases from other filamentous fungi. The coding sequence is interrupted by six introns varying in size from 48 to 98 bp. The intron positions are all conserved among the niaD genes from A. oryzae, Aspergillus nidulans, and Aspergillus niger. A homologous transformation system was developed for an industrial shoyu koji mold, A. oryzae KBN616, based on the nitrate reductase (niaD) of the nitrate assimilation pathway.

摘要

从米曲霉KBN616中分离出编码硝酸还原酶的niaD基因并进行测序。该结构基因由2973个碱基对和868个氨基酸组成，与其他丝状真菌的硝酸还原酶具有高度相似性。编码序列被6个大小从48到98个碱基对不等的内含子打断。这些内含子的位置在米曲霉、构巢曲霉和黑曲霉的niaD基因中都是保守的。基于硝酸同化途径的硝酸还原酶（niaD），为工业酱油曲霉菌株米曲霉KBN616开发了同源转化系统。