从米曲霉KBN616克隆的聚半乳糖醛酸酶基因的结构特征

Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616.

作者信息

Kitamoto N, Kimura T, Kito Y, Ohmiya K, Tsukagoshi N

机构信息

Food Research Institute, Aichi Prefectural Government, Nagoya, Japan.

出版信息

FEMS Microbiol Lett. 1993 Jul 15;111(1):37-41. doi: 10.1111/j.1574-6968.1993.tb06358.x.

DOI:10.1111/j.1574-6968.1993.tb06358.x

PMID:8359678

Abstract

A genomic gene encoding a polygalacturonase from Aspergillus oryzae, used in soy sauce production, was cloned and sequenced. The structural gene comprises 1227 bp coding for 363 amino acids with a putative prepropeptide of 28 amino acids and the open reading frame is disrupted by two short introns of 57 bp and 81 bp. The deduced amino acid sequence of the mature protein showed 63, 63, 63 and 64% homology with those of Aspergillus niger polygalacturonase I, Aspergillus niger polygalacturonase II, Aspergillus tubingensis polygalacturonase II and Cochliobolus carbonum polygalacturonase, respectively. There is, however, little homology among fungal, plant and bacterial polygalacturonases.

摘要

克隆并测序了一种来自米曲霉的多聚半乳糖醛酸酶的基因组基因，该酶用于酱油生产。该结构基因包含1227个碱基对，编码363个氨基酸，带有一个推定的28个氨基酸的前原肽，开放阅读框被两个分别为57个碱基对和81个碱基对的短内含子打断。成熟蛋白的推导氨基酸序列与黑曲霉多聚半乳糖醛酸酶I、黑曲霉多聚半乳糖醛酸酶II、管囊曲霉多聚半乳糖醛酸酶II和禾谷炭疽菌多聚半乳糖醛酸酶的氨基酸序列分别具有63%、63%、63%和64%的同源性。然而，真菌、植物和细菌的多聚半乳糖醛酸酶之间同源性较低。