卵胞浆内单精子注射后未受精卵母细胞的细胞遗传学分析。
Cytogenetic analysis of uncleaved oocytes after intracytoplasmic sperm injection.
作者信息
Bergère M, Selva J, Volante M, Dumont M, Hazout A, Olivennes F, Frydman R
机构信息
Gyneco-obstetric Department, Hôpital Antoine Béclère, Clamart, France.
出版信息
J Assist Reprod Genet. 1995 May;12(5):322-5. doi: 10.1007/BF02213712.
PURPOSE
This work analyzes the causes of cleavage failure after intracytoplasmic sperm injection (ICSI) and the effect of the procedure on the chromosomes of the oocytes.
METHODS
Ninety-seven uncleaved oocytes from 39 patients with severe male infertility or repeated IVF failure were fixed; 79 were analyzable. We checked the decondensation stage of spermatozoa nucleus and the chromosomal abnormalities of the oocytes.
RESULTS
Among the fixed oocytes, the spermatozoa nucleus was present in 97% of the cases, and it was undecondensed in 89% of the cases, showing no evolution at all. A low rate (2.6%) of premature chromosome condensation (PCC) of the spermatozoa and a low rate (2.5%) of female diploïdy were observed. Among the oocytes that could be karyotyped, we observed a high rate (45%) of chromosome breakage.
CONCLUSION
ICSI fertilization failure was due mostly to the complete lack of evolution of the spermatozoa nucleus. Oocyte selection before ICSI seemed to lower the PCC rate. The high rate of oocyte chromosomal breakage rate has to be confirmed.
目的
本研究分析卵胞浆内单精子注射（ICSI）后卵裂失败的原因以及该操作对卵母细胞染色体的影响。
方法
对39例严重男性不育或反复体外受精（IVF）失败患者的97枚未卵裂卵母细胞进行固定；其中79枚可用于分析。我们检查了精子细胞核的去浓缩阶段以及卵母细胞的染色体异常情况。
结果
在固定的卵母细胞中，97%的病例存在精子细胞核，其中89%未发生去浓缩，完全没有变化。观察到精子过早染色体凝聚（PCC）的发生率较低（2.6%），女性二倍体率也较低（2.5%）。在可进行核型分析的卵母细胞中，我们观察到染色体断裂的发生率较高（45%）。
结论
ICSI受精失败主要是由于精子细胞核完全没有变化。ICSI前的卵母细胞选择似乎可降低PCC发生率。卵母细胞染色体断裂率较高这一情况有待进一步证实。
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