Edirisinghe W R, Murch A R, Junk S M, Yovich J L
PIVET Medical Centre, Leederville, Western Australia.
Hum Reprod. 1998 Nov;13(11):3094-8. doi: 10.1093/humrep/13.11.3094.
A man with globozoospermia was treated in our in-vitro fertilization-intracytoplasmic sperm injection (ICSI) programme. In the treatment cycle, 24 oocytes were collected from his wife. All the oocytes were at metaphase II stage. The semen sample produced on the day had a normal sperm count, good motility, but with 100% globozoospermia. All oocytes were injected with randomly selected spermatozoa and of these, two oocytes showed two pronuclei and another contained a single pronucleus. The remainder were unfertilized. The normally fertilized oocytes (two pronuclear) cleaved to the four-cell stage and were transferred to the patient. At 48 h after ICSI, the 21 unfertilized oocytes were processed for cytogenetic analysis. All oocytes contained a haploid chromosome set. The only abnormality seen was a chromosome fragment in one metaphase. Eighteen oocytes contained decondensed sperm nuclei and of these, 14 nuclei were beginning to show signs of premature chromatin condensation (PCC) and the other four showed strong signs of PCC. Thus it appears that in some forms of globozoospermia, arrest of nuclear decondensation and/or PCC are another cause of fertilization failure. The most likely cause for this is the absence or down-regulation of spermatozoa associated activating factor in round-headed spermatozoa.
一名圆头精子症患者在我们的体外受精-卵胞浆内单精子注射（ICSI）项目中接受治疗。在治疗周期中，从他妻子体内采集了24枚卵母细胞。所有卵母细胞均处于减数分裂中期II阶段。当天采集的精液样本精子计数正常、活力良好，但圆头精子症比例为100%。所有卵母细胞均注射了随机选取的精子，其中两枚卵母细胞显示出两个原核，另一枚含有一个原核。其余的未受精。正常受精的卵母细胞（两个原核）分裂至四细胞阶段并移植给患者。ICSI术后48小时，对21枚未受精卵母细胞进行细胞遗传学分析。所有卵母细胞均含有单倍体染色体组。唯一观察到的异常是一个中期细胞中有一条染色体片段。18枚卵母细胞含有解聚的精核，其中14个核开始出现染色质过早凝聚（PCC）迹象，另外4个显示出强烈的PCC迹象。因此，在某些形式的圆头精子症中，核解聚停滞和/或PCC似乎是受精失败的另一个原因。最可能的原因是圆头精子中缺乏或下调了精子相关激活因子。
