小核糖核酸病毒3C蛋白酶：具有丝氨酸蛋白酶折叠结构的半胱氨酸亲核试剂
The picornaviral 3C proteinases: cysteine nucleophiles in serine proteinase folds.
作者信息
Malcolm B A
机构信息
Department of Biochemistry, University of Alberta, Edmonton, Canada.
出版信息
Protein Sci. 1995 Aug;4(8):1439-45. doi: 10.1002/pro.5560040801.
Abstract
The 3C proteinases are a novel group of cysteine proteinases with a serine proteinase-like fold that are responsible for the bulk of polyprotein processing in the Picornaviridae. Because members of this viral family are to blame for several ongoing global pandemic problems (rhinovirus, hepatitis A virus) as well as sporadic outbreaks of more serious pathologies (poliovirus), there has been continuing interest over the last two decades in the development of antiviral therapies. The recent determination of the structure of two of the 3C proteinases by X-ray crystallography opens the door for the application of the latest advances in computer-assisted identification and design of anti-proteinase therapeutic/chemoprophylactic agents.
摘要
3C蛋白酶是一组新型的半胱氨酸蛋白酶，具有类似丝氨酸蛋白酶的折叠结构，负责微小核糖核酸病毒科中大部分多聚蛋白的加工。由于这个病毒家族的成员是造成一些持续的全球大流行问题（鼻病毒、甲型肝炎病毒）以及更严重疾病（脊髓灰质炎病毒）的零星爆发的原因，在过去二十年里，人们一直对开发抗病毒疗法感兴趣。最近通过X射线晶体学确定了两种3C蛋白酶的结构，这为应用计算机辅助鉴定和设计抗蛋白酶治疗/化学预防剂方面的最新进展打开了大门。
相似文献
1
The picornaviral 3C proteinases: cysteine nucleophiles in serine proteinase folds.小核糖核酸病毒3C蛋白酶：具有丝氨酸蛋白酶折叠结构的半胱氨酸亲核试剂
Protein Sci. 1995 Aug;4(8):1439-45. doi: 10.1002/pro.5560040801.
2
Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases.微小核糖核酸病毒3C半胱氨酸蛋白酶具有与胰凝乳蛋白酶样丝氨酸蛋白酶相似的折叠结构。
Nature. 1994 May 5;369(6475):72-6. doi: 10.1038/369072a0.
3
The structures of picornaviral proteinases.微小核糖核酸病毒蛋白酶的结构。
Virus Res. 1999 Aug;62(2):159-68. doi: 10.1016/s0168-1702(99)00043-x.
4
Refined X-ray crystallographic structure of the poliovirus 3C gene product.脊髓灰质炎病毒3C基因产物的精细X射线晶体结构
J Mol Biol. 1997 Nov 14;273(5):1032-47. doi: 10.1006/jmbi.1997.1306.
5
Inhibitors of 3C cysteine proteinases from Picornaviridae.来自小核糖核酸病毒科的3C半胱氨酸蛋白酶抑制剂
Curr Top Med Chem. 2004;4(12):1239-53. doi: 10.2174/1568026043387836.
6
Roles of the Picornaviral 3C Proteinase in the Viral Life Cycle and Host Cells.微小核糖核酸病毒3C蛋白酶在病毒生命周期及宿主细胞中的作用
Viruses. 2016 Mar 17;8(3):82. doi: 10.3390/v8030082.
7
The unusual catalytic triad of poliovirus protease 3C.脊髓灰质炎病毒蛋白酶3C不同寻常的催化三联体。
Biochemistry. 2003 Jan 21;42(2):516-22. doi: 10.1021/bi027004w.
8
Crystal structure of an inhibitor complex of the 3C proteinase from hepatitis A virus (HAV) and implications for the polyprotein processing in HAV.甲型肝炎病毒（HAV）3C蛋白酶抑制剂复合物的晶体结构及其对HAV多蛋白加工的影响
Virology. 1999 Dec 5;265(1):153-63. doi: 10.1006/viro.1999.9968.
9
Expression and purification of recombinant rhinovirus 14 3CD proteinase and its comparison to the 3C proteinase.重组鼻病毒14 3CD蛋白酶的表达、纯化及其与3C蛋白酶的比较。
Arch Biochem Biophys. 1997 Oct 1;346(1):125-30. doi: 10.1006/abbi.1997.0291.
10
Hepatitis A virus 3C proteinase: some properties, crystallization and preliminary crystallographic characterization.甲型肝炎病毒3C蛋白酶：一些特性、结晶及初步晶体学表征
J Mol Biol. 1993 Dec 5;234(3):890-3. doi: 10.1006/jmbi.1993.1636.
引用本文的文献
1
Prioritisation of Compounds for 3CL Inhibitor Development on SARS-CoV-2 Variants.针对 SARS-CoV-2 变异株的 3CL 抑制剂开发进行化合物的优先级排序。
Molecules. 2021 May 18;26(10):3003. doi: 10.3390/molecules26103003.
2
Ensemble Docking Coupled to Linear Interaction Energy Calculations for Identification of Coronavirus Main Protease (3CL) Non-Covalent Small-Molecule Inhibitors.基于对接和线性相互作用能计算的冠状病毒主蛋白酶（3CL）非共价小分子抑制剂的筛选。
Molecules. 2020 Dec 9;25(24):5808. doi: 10.3390/molecules25245808.
3
Characterization of Self-Processing Activities and Substrate Specificities of Porcine Torovirus 3C-Like Protease.猪传染性胃肠炎病毒 3C 样蛋白酶的自我加工活性和底物特异性的表征。
J Virol. 2020 Sep 29;94(20). doi: 10.1128/JVI.01282-20.
4
Homology Models and Molecular Dynamics Simulations of Main Proteinase from Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS).严重急性呼吸综合征（SARS）冠状病毒主要蛋白酶的同源模型与分子动力学模拟
J Chin Chem Soc. 2004 Oct;51(5A):889-900. doi: 10.1002/jccs.200400134. Epub 2013 Sep 25.
5
Viral cysteine proteinases.病毒半胱氨酸蛋白酶
Perspect Drug Discov Des. 1996;6(1):64-86. doi: 10.1007/BF02174046.
6
Structural basis for catalysis and substrate specificity of a 3C-like cysteine protease from a mosquito mesonivirus.一种蚊虫 M 型弹状病毒 3C 样半胱氨酸蛋白酶的催化和底物特异性的结构基础。
Virology. 2019 Jul;533:21-33. doi: 10.1016/j.virol.2019.05.001. Epub 2019 May 2.
7
Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights.柯萨奇病毒B3蛋白酶3C：表达、纯化、结晶及初步结构解析
Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):877-884. doi: 10.1107/S2053230X16018513. Epub 2016 Nov 25.
8
Recent Advances in the Discovery of Norovirus Therapeutics.诺如病毒治疗方法发现的最新进展
J Med Chem. 2015 Dec 24;58(24):9438-50. doi: 10.1021/acs.jmedchem.5b00762. Epub 2015 Aug 17.
9
The enterovirus 3C protease inhibitor SG85 efficiently blocks rhinovirus replication and is not cross-resistant with rupintrivir.肠道病毒3C蛋白酶抑制剂SG85能有效阻断鼻病毒复制，且与鲁平替尼无交叉耐药性。
Antimicrob Agents Chemother. 2015 Sep;59(9):5814-8. doi: 10.1128/AAC.00534-15. Epub 2015 Jun 8.
10
Structural basis for host membrane remodeling induced by protein 2B of hepatitis A virus.甲型肝炎病毒蛋白2B诱导宿主细胞膜重塑的结构基础
J Virol. 2015 Apr;89(7):3648-58. doi: 10.1128/JVI.02881-14. Epub 2015 Jan 14.
本文引用的文献
1
Anti-malarial drug development using models of enzyme structure.利用酶结构模型进行抗疟药物研发。
Chem Biol. 1994 Sep;1(1):31-7. doi: 10.1016/1074-5521(94)90038-8.
2
Structure-based inhibitor design by using protein models for the development of antiparasitic agents.利用蛋白质模型进行基于结构的抑制剂设计以开发抗寄生虫药物。
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3583-7. doi: 10.1073/pnas.90.8.3583.
3
Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes.病毒编码蛋白酶的表达：与细胞酶的功能和结构相似性
Microbiol Rev. 1993 Dec;57(4):781-822. doi: 10.1128/mr.57.4.781-822.1993.
4
Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA-binding site, and means for cleaving precursor polyprotein.人鼻病毒3C蛋白酶的结构揭示了一种胰蛋白酶样多肽折叠、RNA结合位点以及切割前体多聚蛋白的方式。
Cell. 1994 Jun 3;77(5):761-71. doi: 10.1016/0092-8674(94)90059-0.
5
On the size of the active site in proteases. I. Papain.关于蛋白酶活性位点的大小。I. 木瓜蛋白酶。
Biochem Biophys Res Commun. 1967 Apr 20;27(2):157-62. doi: 10.1016/s0006-291x(67)80055-x.
6
Role of a buried acid group in the mechanism of action of chymotrypsin.一个埋藏的酸性基团在胰凝乳蛋白酶作用机制中的作用。
Nature. 1969 Jan 25;221(5178):337-40. doi: 10.1038/221337a0.
7
Crystal and molecular structures of the complex of alpha-chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 A resolution.α-胰凝乳蛋白酶与其抑制剂火鸡卵类粘蛋白第三结构域复合物的晶体结构及分子结构，分辨率为1.8埃
J Mol Biol. 1987 May 20;195(2):397-418. doi: 10.1016/0022-2836(87)90659-0.
8
Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications.病毒半胱氨酸蛋白酶与丝氨酸蛋白酶的胰蛋白酶样家族同源：结构和功能意义。
Proc Natl Acad Sci U S A. 1988 Nov;85(21):7872-6. doi: 10.1073/pnas.85.21.7872.
9
Viral proteinases.病毒蛋白酶
Annu Rev Biochem. 1988;57:701-54. doi: 10.1146/annurev.bi.57.070188.003413.
10
Site-directed mutagenesis of proteinase 3C results in a poliovirus deficient in synthesis of viral RNA polymerase.蛋白酶3C的定点诱变导致脊髓灰质炎病毒缺乏病毒RNA聚合酶的合成。
J Virol. 1987 Jul;61(7):2162-70. doi: 10.1128/JVI.61.7.2162-2170.1987.
Zotero 插件