来自大肠杆菌的重组L-核糖-5-磷酸4-表异构酶的纯化及初步X射线晶体学研究
Purification and preliminary X-ray crystallographic studies of recombinant L-ribulose-5-phosphate 4-epimerase from Escherichia coli.
作者信息
Andersson A, Schneider G, Lindqvist Y
机构信息
Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Sweden.
出版信息
Protein Sci. 1995 Aug;4(8):1648-50. doi: 10.1002/pro.5560040823.
Abstract
The araD gene from Escherichia coli, coding for L-ribulose-5-phosphate 4-epimerase, was overexpressed and the resulting enzyme was purified to homogeneity. Crystals of L-ribulose-5-phosphate 4-epimerase, obtained with 4.0 M sodium formate as precipitant, belong to space group P4212 with unit cell dimensions a = b = 107.8 A and c = 281.4 A and diffract to at least 2.2 A resolution. Density measurements of these crystals are consistent with eight subunits in the asymmetric unit.
摘要
来自大肠杆菌的编码L-核糖-5-磷酸4-表异构酶的araD基因被过量表达，产生的酶被纯化至同质。以4.0 M甲酸钠作为沉淀剂获得的L-核糖-5-磷酸4-表异构酶晶体属于空间群P4212，晶胞参数a = b = 107.8 Å，c = 281.4 Å，衍射分辨率至少为2.2 Å。这些晶体的密度测量结果与不对称单元中的八个亚基一致。
相似文献
1
Purification and preliminary X-ray crystallographic studies of recombinant L-ribulose-5-phosphate 4-epimerase from Escherichia coli.来自大肠杆菌的重组L-核糖-5-磷酸4-表异构酶的纯化及初步X射线晶体学研究
Protein Sci. 1995 Aug;4(8):1648-50. doi: 10.1002/pro.5560040823.
2
Structural study of Escherichia coli NAD synthetase: overexpression, purification, crystallization, and preliminary crystallographic analysis.大肠杆菌烟酰胺腺嘌呤二核苷酸合成酶的结构研究：过表达、纯化、结晶及初步晶体学分析。
J Struct Biol. 1999 Oct;127(3):279-82. doi: 10.1006/jsbi.1999.4152.
3
Purification, crystallization, and preliminary X-ray crystallographic data analysis of small heat shock protein homolog from Methanococcus jannaschii, a hyperthermophile.嗜热古菌詹氏甲烷球菌小分子热激蛋白同源物的纯化、结晶及初步X射线晶体学数据分析
J Struct Biol. 1998 Jan;121(1):76-80. doi: 10.1006/jsbi.1998.3969.
4
Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii.刚地弓形虫尿嘧啶磷酸核糖转移酶的表达、纯化、结晶及初步X射线衍射分析
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):347-9. doi: 10.1107/S090744499800821X. Epub 1999 Jan 1.
5
Preliminary crystallographic studies of Y25F mutant of periplasmic Escherichia coli L-asparaginase.
Acta Biochim Pol. 2000;47(3):807-14.
6
Glutamate-5-kinase from Escherichia coli: gene cloning, overexpression, purification and crystallization of the recombinant enzyme and preliminary X-ray studies.来自大肠杆菌的谷氨酸-5-激酶：重组酶的基因克隆、过量表达、纯化与结晶及初步X射线研究
Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2091-4. doi: 10.1107/S0907444904023972. Epub 2004 Oct 20.
7
Crystallization and characterization of a fragment of pseudouridine synthase RluC from Escherichia coli.来自大肠杆菌的假尿苷合酶RluC片段的结晶与表征
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):302-4. doi: 10.1107/S090744499801021X. Epub 1999 Jan 1.
8
Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1.酵母磷酸化传递蛋白YPD1的纯化、结晶及初步X射线衍射分析
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):291-3. doi: 10.1107/S090744499800866X. Epub 1999 Jan 1.
9
The isolation, purification, and preliminary crystallographic characterization of UDP-galactose-4-epimerase from Escherichia coli.来自大肠杆菌的UDP-半乳糖-4-表异构酶的分离、纯化及初步晶体学表征
Proteins. 1991;9(2):135-42. doi: 10.1002/prot.340090207.
10
Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile.嗜热古菌詹氏甲烷球菌中一种纤维原蛋白同源物的表达、纯化及初步X射线分析
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):338-40. doi: 10.1107/S0907444998007513. Epub 1999 Jan 1.
引用本文的文献
1
Identification and analysis of the paulomycin biosynthetic gene cluster and titer improvement of the paulomycins in Streptomyces paulus NRRL 8115.保罗霉素生物合成基因簇的鉴定与分析以及提高保罗链霉菌NRRL 8115中保罗霉素的产量
PLoS One. 2015 Mar 30;10(3):e0120542. doi: 10.1371/journal.pone.0120542. eCollection 2015.
2
Utilization of D-ribitol by Lactobacillus casei BL23 requires a mannose-type phosphotransferase system and three catabolic enzymes.干酵母醇（D-ribitol）被干酪乳杆菌 BL23 利用需要一个甘露糖型磷酸转移酶系统和三种代谢酶。
J Bacteriol. 2013 Jun;195(11):2652-61. doi: 10.1128/JB.02276-12. Epub 2013 Apr 5.
本文引用的文献
1
Pentose fermenation by Lactobacillus plantarum. IV. L-ribulose-5-phosphate 4-epimerase.植物乳杆菌的戊糖发酵。IV. L-核糖-5-磷酸4-表异构酶。
J Biol Chem. 1958 Apr;231(2):1053-64.
2
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
3
Crystalline L-ribulose 5-phosphate 4-epimerase from Escherichia coli.来自大肠杆菌的结晶型L-核糖-5-磷酸4-表异构酶。
J Biol Chem. 1968 Sep 25;243(18):4700-5.
4
Determination of the protein content of crystals formed by Mastigocladus laminosus C-phycocyanin, Chroomonas spec. phycocyanin-645 and modified human fibrinogen using an improved Ficoll density gradient method.使用改进的Ficoll密度梯度法测定层状鞭枝藻C-藻蓝蛋白、嗜色藻属藻蓝蛋白-645和修饰的人纤维蛋白原形成的晶体的蛋白质含量。
Biol Chem Hoppe Seyler. 1985 Mar;366(3):287-95. doi: 10.1515/bchm3.1985.366.1.287.
5
The organization of the araBAD operon of Escherichia coli.大肠杆菌araBAD操纵子的组织形式。
Gene. 1986;47(2-3):231-44. doi: 10.1016/0378-1119(86)90067-3.
6
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.
7
Purification and properties of D-ribulose-5-phosphate 3-epimerase from calf liver.小牛肝脏中D-核糖-5-磷酸3-表异构酶的纯化及性质
Biochim Biophys Acta. 1979 Oct 11;570(2):352-62. doi: 10.1016/0005-2744(79)90155-4.
8
DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.
Zotero 插件