Tieppo C A, Silva A M, Palermo-Neto J, Nasello A G, Felicio L F
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1995 Mar;28(3):351-4.
Cholecystokinin (CCK-8) coexists with dopamine in some neurons and modulates dopaminergic neurotransmission. In the present study we determined the effect of CCK-8 on stereotyped behavior in supersensitive dopaminergic system. Adult male Wistar rats, weighing 200-250 g, were used. Dopaminergic supersensitivity was induced by long-term haloperidol (HAL) treatment (30 days: 1.0 mg/kg twice a day). Seventy-two hours after HAL withdrawal animals received CCK-8 (14.5 nmol/5 microliters) or saline intracerebroventricularly (icv) before being tested for apomorphine (APO, 0.6 mg/kg, sc)-induced stereotyped behavior. Experimental groups were: long-term HAL-treated rats that received saline (HSAL, N = 9) or CCK-8 (HCCK, N = 11) icv and long-term saline-treated rats that received CCK-8 (SCCK, N = 9) or saline (SSAL, N = 8) icv. As expected, HSAL rats showed statistically significant higher stereotypy scores than SSAL rats (42 +/- 1.7 vs 31 +/- 1.6; P < 0.05) and CCK-8 significantly reduced stereotyped behavior in supersensitive rats (42 +/- 1.7 vs 37 +/- 1.5; P < 0.05). These results show that CCK-8 icv reduces stereotypy in dopaminergic-supersensitive rats, and suggest that the dopamine supersensitivity phenomenon can be modulated by CCK-8.
胆囊收缩素（CCK - 8）在一些神经元中与多巴胺共存，并调节多巴胺能神经传递。在本研究中，我们确定了CCK - 8对超敏多巴胺能系统中刻板行为的影响。使用体重为200 - 250克的成年雄性Wistar大鼠。通过长期氟哌啶醇（HAL）治疗（30天：每天两次，每次1.0毫克/千克）诱导多巴胺能超敏反应。在停止使用HAL 72小时后，动物在接受阿扑吗啡（APO，0.6毫克/千克，皮下注射）诱导的刻板行为测试前，经脑室内（icv）注射CCK - 8（14.5纳摩尔/5微升）或生理盐水。实验组包括：长期接受HAL治疗并经icv注射生理盐水的大鼠（HSAL，N = 9）或CCK - 8（HCCK，N = 11），以及长期接受生理盐水治疗并经icv注射CCK - 8（SCCK，N = 9）或生理盐水（SSAL，N = 8）的大鼠。正如预期的那样，HSAL大鼠的刻板行为评分在统计学上显著高于SSAL大鼠（42±1.7对31±1.6；P < 0.05），并且CCK - 8显著降低了超敏大鼠的刻板行为（42±1.7对37±1.5；P < 0.05）。这些结果表明，脑室内注射CCK - 8可降低多巴胺能超敏大鼠的刻板行为，并提示多巴胺超敏现象可被CCK - 8调节。
