精子制动抗体对自发及钙离子载体（A23187）诱导的顶体反应的影响。

Effect on sperm-immobilizing antibodies on the spontaneous and calcium-ionophore (A23187)-induced acrosome reaction.

作者信息

Tasdemir I, Tasdemir M, Fukuda J, Kodama H, Matsui T, Tanaka T

机构信息

Department of Obstetrics and Gynecology Akita University School of Medicine, Japan.

出版信息

Int J Fertil Menopausal Stud. 1995 Jul-Aug;40(4):192-5.

PMID:8520620

Abstract

OBJECTIVE

To study the effect of sperm-immobilizing antibodies on spontaneous and A23187-induced acrosome reactions.

MATERIAL AND METHODS

Swim-up spermatozoa obtained from a fertile donor were incubated with 16 sera containing sperm-immobilizing antibodies obtained from infertile women and 3 control sera obtained from healthy females. The acrosome loss, either occurring spontaneously or induced by 10-microM A23181, were examined by staining sperm with Pisum sativum agglutinin labeled with fluorescein isothiocyanate.

RESULTS

The incidence of the spontaneous acrosome reaction of spermatozoa incubated with anti-sperm antibodies (6.25 +/- 1.4%) was significantly (P < .001) lower than that of the spermatozoa incubated with control sera (12 +/- 0.8%). The incidence of A23187-induced and inducible (incidence of induced minus spontaneous) acrosome reactions of spermatozoa incubated with sera-positive for sperm antibodies (10.4 +/- 1.3% and 4.2 +/- 1.5%) was also significantly lower (P < .001) than that of spermatozoa incubated with control sera (30.7 +/- 0.5% and 18.7 +/- 1.2%).

CONCLUSION

Sperm immobilizing antibodies interfere with fertilization by inhibiting the acrosome reaction.

摘要

目的

研究精子制动抗体对自发及A23187诱导的顶体反应的影响。

材料与方法

将从一名生育能力正常的供体获取的上游精子与16份来自不孕女性的含精子制动抗体的血清以及3份来自健康女性的对照血清一起孵育。通过用异硫氰酸荧光素标记的豌豆凝集素对精子进行染色，检测自发发生或由10微摩尔A23181诱导发生的顶体丢失情况。

结果

与抗精子抗体孵育的精子的自发顶体反应发生率（6.25±1.4%）显著低于（P<.001）与对照血清孵育的精子（12±0.8%）。与精子抗体呈阳性的血清孵育的精子的A23187诱导及可诱导（诱导发生率减去自发发生率）顶体反应发生率（10.4±1.3%和4.2±1.5%）也显著低于（P<.001）与对照血清孵育的精子（30.7±0.5%和18.7±1.2%）。