Taşdemir I, Taşdemir M, Fukuda J, Kodama H, Matsui T, Tanaka T
Department of Microbiology, Ege University School of Medicine, Bornova, Izmir, Turkey.
J Assist Reprod Genet. 1996 May;13(5):413-6. doi: 10.1007/BF02066174.
To study the effect of sperm-immobilizing antibodies from male sera on spontaneous and A23187-induced acrosome reactions (AR).
Swim-up spermatozoa obtained from three fertile donors were incubated with 13 sera with sperm-immobilizing antibodies obtained from infertile men and three control sera obtained from healthy fertile males. Sperm acrosomes were examined by staining with pisum sativum agglutinin labeled with fluorescein isothiocyanate (30 micrograms/ml; Sigma Chemical Co., St. Louis. MO) as spontaneous and A23187 (used at a final concentration of 10 microM; Sigma Chemical Co.) induced.
The incidence of spontaneous AR of spermatozoa incubated with antisperm antibody positive male sera (6.2 +/- 0.7) was significantly (P < 0.001) lower than that of spermatozoa incubated with control sera (10.7 +/- 0.5). And the incidence of A23187-induced and -inducible (incidence of induced minus spontaneous) ARs of spermatozoa incubated with sperm antibody-positive male sera (12.4 +/- 1.9 and 6.2 +/- 1.9) was significantly lower (P < 0.001) than that of spermatozoa incubated with control sera (31.0 +/- 0.5 and 20.3 +/- 0.9). Sperm-immobilizing antibody-positive sera decreased spontaneous, A23187-induced, and inducible ARs.
Sperm-immobilizing antibodies from male sera interfere with fertilization by inhibiting the AR.
研究男性血清中精子制动抗体对自发及A23187诱导的顶体反应（AR）的影响。
从三名生育能力正常的供体获取的上游精子，与从不育男性获得的13份含精子制动抗体的血清以及从健康有生育能力男性获得的3份对照血清一起孵育。通过用异硫氰酸荧光素标记的豌豆凝集素（30微克/毫升；西格玛化学公司，密苏里州圣路易斯）染色来检测精子顶体，分别检测自发及A23187（终浓度为10微摩尔；西格玛化学公司）诱导的情况。
与抗精子抗体阳性男性血清孵育的精子自发AR发生率（6.2±0.7）显著低于（P<0.001）与对照血清孵育的精子（10.7±0.5）。与精子抗体阳性男性血清孵育的精子A23187诱导及诱导性（诱导发生率减去自发发生率）AR发生率（12.4±1.9和6.2±1.9）显著低于（P<0.001）与对照血清孵育的精子（31.0±0.5和20.3±0.9）。精子制动抗体阳性血清降低了自发、A23187诱导及诱导性AR。
男性血清中的精子制动抗体通过抑制顶体反应干扰受精。
