Witko-Sarsat V, Delacourt C, Rabier D, Bardet J, Nguyen A T, Descamps-Latscha B
INSERM U25, Hôpital Necker, Paris, France.
Am J Respir Crit Care Med. 1995 Dec;152(6 Pt 1):1910-6. doi: 10.1164/ajrccm.152.6.8520754.
We evaluated long-lived oxidant potential in the sputum of patients with cystic fibrosis (CF) by quantitating the methionine-inhibitable, long-lived oxidant fraction of sputum, referred to as the chloramines. Taurine, the preferred amino acid substrate for chloramine formation, and myeloperoxidase (MPO), the chlorinated oxidant-generating enzyme, were also quantitated. As compared with the sputum of asthmatic subjects, the sputum of CF patients contained high concentrations of chloramines along with high levels of taurine and active MPO. A negative correlation between chloramine and taurine was found in the sputum of CF patients. No correlation was found between the density of Pseudomonas aeruginosa and the level of chloramines, taurine, or MPO. In contrast, respiratory parameters (%FEV or %FVC) and a nutritional index correlated positively with chloramine levels, whereas negative correlations were observed with taurine and MPO. In addition, the effect of antibiotic therapy, which significantly increased chloramine and decreased taurine levels, supported a beneficial effect of chloramines on overall clinical status. Our findings support a dual role of long-lived oxidants at the site of airway inflammation in CF, one component of which is their ability to mediate oxidative stress and the other a beneficial effect that may be partly explained by their inhibitory effect on antiprotease defense systems.
我们通过对痰液中蛋氨酸可抑制的长寿命氧化剂部分（即氯胺）进行定量，评估了囊性纤维化（CF）患者痰液中的长寿命氧化剂潜力。还对氯胺形成的首选氨基酸底物牛磺酸以及产生氯化氧化剂的酶髓过氧化物酶（MPO）进行了定量。与哮喘患者的痰液相比，CF患者的痰液中含有高浓度的氯胺以及高水平的牛磺酸和活性MPO。在CF患者的痰液中发现氯胺与牛磺酸呈负相关。铜绿假单胞菌的密度与氯胺、牛磺酸或MPO的水平之间未发现相关性。相反，呼吸参数（%FEV或%FVC）和营养指数与氯胺水平呈正相关，而与牛磺酸和MPO呈负相关。此外，抗生素治疗显著增加了氯胺并降低了牛磺酸水平，这支持了氯胺对整体临床状况的有益作用。我们的研究结果支持长寿命氧化剂在CF气道炎症部位的双重作用，其中一个作用是它们介导氧化应激的能力，另一个有益作用可能部分由它们对抗蛋白酶防御系统的抑制作用来解释。
