Lundberg B
Chem Phys Lipids. 1977 Mar;18(2):212-20. doi: 10.1016/0009-3084(77)90008-1.
The cholesterol solubilizing capacity of lecithin vesicles was studied and some physicochemical properties of the resulting mixed vesicles were investigated. The maximum association of ultrasonicated cholesterol and lecithin was found to be a cholesterol/lecithin molar ratio of 2:1, with a limiting concentration of colloidal lipid of approximately 34 mg/ml. The 2:1 dispersions were found to be rather stable with no change in cholesterol/lecithin ratios for long periods. The mixed 2:1 cholesterol/lecithin vesicles were separated by Sepharose 4 B chromatography to obtain homogeneous preparations. The homogeneity was further tested by analytical ultracentrifugation and electron microscopy. Light-scattering measurements showed an increase in particle weight with increasing cholesterol proportion. 1H-and 13C-NMR studies demonstrated an additional broadening, especially of chain resonances, when going from a cholesterol/lecithin molar ratio of 1:1 to 2:1.
研究了卵磷脂囊泡的胆固醇溶解能力，并对所得混合囊泡的一些物理化学性质进行了研究。发现超声处理的胆固醇和卵磷脂的最大缔合是胆固醇/卵磷脂摩尔比为2:1，胶体脂质的极限浓度约为34mg/ml。发现2:1的分散体相当稳定，胆固醇/卵磷脂比率长时间不变。通过琼脂糖4B色谱法分离2:1胆固醇/卵磷脂混合囊泡，以获得均匀制剂。通过分析超速离心和电子显微镜进一步测试均匀性。光散射测量表明，随着胆固醇比例的增加，颗粒重量增加。1H和13C核磁共振研究表明，当胆固醇/卵磷脂摩尔比从1:1变为2:1时，会出现额外的谱线加宽，尤其是链共振。
