Brunner J, Skrabal P, Hauser H
Biochim Biophys Acta. 1976 Dec 2;455(2):322-31. doi: 10.1016/0005-2736(76)90308-4.
Single shelled lecithin vesicles of uniform size (diameter = 300 A) are prepared without sonication by solubilizing unsonicated lecithin dispersions with sodium cholate and removing the detergent from the mixed lecithin - cholate micelles by gel filtration on Sephadex G-50. A homogeneous population of pure lecithin single-bilayer vesicles free of multilamellar structures is obtained. The vesicle diameter is somewhat larger than the average diameter of sonicated vesicles. The curvature of the bilayer seems to be sufficiently large to allow for similar packing densities (areas/molecule) on the outer and inner layer of the bilayer. The morphology and some physico-chemical properties of these vesicles are described and compared with those of sonicated vesicles.
通过用胆酸钠溶解未超声处理的卵磷脂分散体，并在Sephadex G - 50上进行凝胶过滤从混合的卵磷脂 - 胆酸盐胶束中去除去污剂，制备出尺寸均匀（直径 = 300 Å）的单壳卵磷脂囊泡，且无需超声处理。获得了不含多层结构的纯卵磷脂单双层囊泡的均匀群体。囊泡直径比超声处理后的囊泡平均直径稍大。双层膜的曲率似乎足够大，以允许双层膜外层和内层具有相似的堆积密度（面积/分子）。描述了这些囊泡的形态和一些物理化学性质，并与超声处理后的囊泡进行了比较。
