Claros M G
Ecole Normale Supérieure, Laboratoire de Génétique Moléculaire, CNRS URA 1302, Paris, France.
Comput Appl Biosci. 1995 Aug;11(4):441-7. doi: 10.1093/bioinformatics/11.4.441.
The paper describes the Macintosh program MitoProt, which is suitable for studying mitochondrion-related proteins. MitoProt supplies a series of parameters that permit theoretical evaluation of mitochondrial targeting sequences, as well as calculation of the most hydrophobic fragment of 17 residues in the sequence, and a new parameter called mesohydrophobicity. The last two calculations are important for predicting the putative importability of a protein into mitochondria. Taken together, targeting sequence and hydrophobicity characteristics enable one to predict whether a given protein could be mitochondrial when no previous information on the nature of the sequence is available.
该论文描述了适用于研究线粒体相关蛋白质的Macintosh程序MitoProt。MitoProt提供了一系列参数,可对线粒体靶向序列进行理论评估,计算序列中17个残基的最疏水片段,以及一个称为中疏水性的新参数。后两项计算对于预测蛋白质导入线粒体的可能性很重要。综合来看,靶向序列和疏水性特征能够在没有关于序列性质的先前信息时,预测给定蛋白质是否可能定位于线粒体。