Filipe P M, Freitas J P, Castro M, Pinto R, Fernandes A, de Castro L C, Rico T, Rodrigo F G
Clínica Dermatológica Universitaŕia, Lisboa.
C R Seances Soc Biol Fil. 1995;189(3):443-51.
UV irradiation induces lipid peroxidation (LPO) and cell damage. The aim of the present work was the study of UVB radiation effects on cultured human skin fibroblasts, concerning LPO, prostaglandine E2 (PGE2) formation and cell viability. The cells were exposed to 50, 100, and 150 mJ/cm2 of UVB irradiation. Cellular TBARS and supernatant fluorescent substances were measured spectrofluorimetrically. PGE2 was measured using an immunoenzymatic method. Cell viability was evaluated by the MTT test. All determinations were done after a 2 h incubation period post-irradiation. TBARS were increased for all doses of irradiation (p < 0.001). Fluorescent substances differed from controls at 50 mJ/cm2 (p < 0.001). UVB at 100 and 150 mJ/cm2 decreased cellular viability (p < 0.001). An increase of PGE2 was observed with UVB at 150 mJ/cm2 (p < 0.001). These results confirm the occurrence of LPO and cytotoxicity after UV irradiation; on the other hand, this study showed the formation of PGE2 induced by UV light on cultured human skin fibroblasts. We propose a relationship between these phenomena.
紫外线照射会诱导脂质过氧化（LPO）和细胞损伤。本研究的目的是研究紫外线B（UVB）辐射对培养的人皮肤成纤维细胞的影响，涉及LPO、前列腺素E2（PGE2）的形成和细胞活力。将细胞暴露于50、100和150 mJ/cm2的UVB辐射下。用荧光分光光度法测量细胞中的硫代巴比妥酸反应物（TBARS）和上清液中的荧光物质。使用免疫酶法测量PGE2。通过MTT试验评估细胞活力。所有测定均在照射后2小时的孵育期后进行。所有照射剂量下的TBARS均增加（p < 0.001）。50 mJ/cm2时的荧光物质与对照组不同（p < 0.001）。100和150 mJ/cm2的UVB降低了细胞活力（p < 0.001）。在150 mJ/cm2的UVB照射下观察到PGE2增加（p < 0.001）。这些结果证实了紫外线照射后LPO的发生和细胞毒性；另一方面，本研究表明紫外线可诱导培养的人皮肤成纤维细胞形成PGE2。我们提出了这些现象之间的一种关系。
