Kondo S, Mamada A, Yamaguchi J, Fukuro S
Department of Dermatology, School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Photodermatol Photoimmunol Photomed. 1990 Aug;7(4):173-7.
The effect of dl-alpha-tocopherol on ultraviolet light, 280-320 nm (UVB)-induced damage of human skin fibroblasts was studied by measuring the colony-forming ability, unscheduled DNA synthesis (UDS) and malondialdehyde (MDA) production. Regarding the cell toxicity, the values of the mean lethal dose (D0) of UV in fibroblast strains from 5 normal subjects were examined. D0 increased dose-dependently when the cells were cultured in the presence of dl-alpha-tocopherol at the concentration of 10-1000 micrograms/ml. UDS induced by 500 J/m2 UVB irradiation was not altered by treatment of 100 micrograms/ml dl-alpha-tocopherol. MDA did not increase after 500 J/m2 UVB irradiation in the fibroblasts cultured with 100 micrograms/ml dl-alpha-tocopherol, while MDA in the fibroblasts cultured without dl-alpha-tocopherol increased after irradiation. These results suggest that dl-alpha-tocopherol protects human skin fibroblasts against the cytotoxic effect of UVB, and its mechanism seems to be related to inhibition of UV-induced lipid peroxidation or to the antioxidation effect of dl-alpha-tocopherol.
通过测量集落形成能力、非程序性DNA合成（UDS）和丙二醛（MDA）生成量，研究了dl-α-生育酚对280 - 320纳米紫外线（UVB）诱导的人皮肤成纤维细胞损伤的影响。关于细胞毒性，检测了5名正常受试者的成纤维细胞株中紫外线的平均致死剂量（D0）值。当细胞在浓度为10 - 1000微克/毫升的dl-α-生育酚存在下培养时，D0呈剂量依赖性增加。用100微克/毫升dl-α-生育酚处理后，500焦/平方米UVB照射诱导的UDS未发生改变。在用100微克/毫升dl-α-生育酚培养的成纤维细胞中，500焦/平方米UVB照射后MDA没有增加，而在没有dl-α-生育酚培养的成纤维细胞中，照射后MDA增加。这些结果表明，dl-α-生育酚可保护人皮肤成纤维细胞免受UVB的细胞毒性作用，其机制似乎与抑制紫外线诱导的脂质过氧化或dl-α-生育酚的抗氧化作用有关。
