Hsu J J, Hsieh T T, Lo L M, Soong Y K
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan, ROC.
Changgeng Yi Xue Za Zhi. 1995 Sep;18(3):240-7.
In order to establish normative median values of maternal serum total human chorionic gonadotropin (hCG) at our own laboratory, 2711 normal sera were collected from uncomplicated, singleton Chinese pregnant women, including 1705 samples measured by radioimmunoassay (RIA) between 13 and 24 weeks' gestation and 1006 samples measured by enzyme immunoassay (EIA) between 14 and 20 weeks' gestation. The hCG secretion pattern throughout midtrimester measured by RIA is similar to that of measured by both RIA and EIA have a steep drop initially and then steadily decline from 17 weeks of gestation. The total (hCG levels measured by RIA and EIA showed different normative median values and distribution. If it was measured by RIA between 14 and 20 week's gestation, there were 3.7% and 17.8% of pregnancies with serum total hCG levels less than 0.25 multiple of the median (MoM) and 0.5 MoM, retrospectively, and 11.6% and 5.5% among them with serum levels above 2.0 MoM and 2.5 MoM, respectively. When it was measured by EIA, there were 0.7% and 9.2% of pregnancies with serum total hCG levels less than 0.25 MoM and 0.5 MoM, respectively, and 8.9% and 3.7% among them with serum levels above 2.0 MoM and 2.5 MoM, respectively. Accurate and satisfactory interpretation of maternal serum screening for Down syndrome depends on establishment of a well-developed normative median value for each week of gestation. Any laboratory intends to provide hCG for maternal serum screening should have its own reference data by its own immunoassay method.
为了在我们自己的实验室建立孕妇血清总人绒毛膜促性腺激素（hCG）的标准中位数，从无并发症的单胎中国孕妇中收集了2711份正常血清，其中包括1705份在妊娠13至24周期间通过放射免疫分析（RIA）测量的样本，以及1006份在妊娠14至20周期间通过酶免疫分析（EIA）测量的样本。通过RIA测量的整个孕中期hCG分泌模式与通过RIA和EIA测量的相似，最初急剧下降，然后从妊娠17周开始稳步下降。RIA和EIA测量的总hCG水平显示出不同的标准中位数和分布。如果在妊娠14至20周期间通过RIA测量，回顾性分析显示，分别有3.7%和17.8%的孕妇血清总hCG水平低于中位数的0.25倍（MoM）和0.5 MoM，其中分别有11.6%和5.5%的孕妇血清水平高于2.0 MoM和2.5 MoM。当通过EIA测量时，分别有0.7%和9.2%的孕妇血清总hCG水平低于0.25 MoM和0.5 MoM，其中分别有8.9%和3.7%的孕妇血清水平高于2.0 MoM和2.5 MoM。对唐氏综合征孕妇血清筛查的准确和满意解释取决于为妊娠的每一周建立完善的标准中位数。任何打算为孕妇血清筛查提供hCG的实验室都应该通过自己的免疫分析方法拥有自己的参考数据。
