Braga P C, Dal Sasso M, Maci S, Allegra L, Fonti E, Ghessi A, Reggio S
Department of Pharmacology, University of Milan, Italy.
Chemotherapy. 1995 Sep-Oct;41(5):360-7. doi: 10.1159/000239368.
Antibiotics not only reach the site of infection, but also penetrate cyclically, during a treatment, into polymorphonuclear leukocytes (PMNs) and may influence their functions positively or negatively. With reference to these aspects, the influence of brodimoprim (BMP), a dimethoxybenzylpyrimidine recently entered into clinical use, on human PMN phagocytosis and oxidant radical production (chemiluminescence) was investigated. PMNs from healthy adult donors were incubated for 50 min in medium alone or in medium containing increasing concentrations (3.7, 7.5, 15, and 30 micrograms/ml) of BMP and trimethoprim (TMP). In unwashed PMNs, phagocytosis was not modified by BMP, but was significantly reduced by 30 micrograms/ml TMP; chemiluminescence was significantly reduced by 15 and 30 micrograms/ml BMP and by all concentrations of TMP. When PMNs were washed after incubation, phagocytosis was unaffected and chemiluminescence was significantly restored. BMP at therapeutic concentrations did not influence PMNs and was less toxic than TMP.
抗生素不仅能到达感染部位，而且在治疗过程中还会周期性地渗透到多形核白细胞（PMN）中，并可能对其功能产生积极或消极的影响。关于这些方面，研究了最近进入临床使用的二甲氧基苄基嘧啶溴莫普明（BMP）对人PMN吞噬作用和氧化剂自由基产生（化学发光）的影响。将健康成年供体的PMN在单独的培养基中或在含有浓度递增（3.7、7.5、15和30微克/毫升）的BMP和甲氧苄啶（TMP）的培养基中孵育50分钟。在未洗涤的PMN中，BMP未改变吞噬作用，但30微克/毫升的TMP显著降低了吞噬作用；15和30微克/毫升的BMP以及所有浓度的TMP均显著降低了化学发光。孵育后洗涤PMN时，吞噬作用未受影响，化学发光显著恢复。治疗浓度的BMP不影响PMN，且毒性低于TMP。
