在枯草芽孢杆菌营养细胞中，gcaD（tms）基因（编码N-乙酰葡糖胺1-磷酸尿苷转移酶）、prs基因（编码磷酸核糖焦磷酸合成酶）和ctc的三顺反子操纵子表达。
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
作者信息
Hilden I, Krath B N, Hove-Jensen B
机构信息
Department of Biological Chemistry, University of Copenhagen, Denmark.
出版信息
J Bacteriol. 1995 Dec;177(24):7280-4. doi: 10.1128/jb.177.24.7280-7284.1995.
Abstract
The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front of the gcaD gene. Analysis of cDNA molecules prepared with gcaD-prs-ctc-specified mRNA as the template revealed an RNA transcript that encompassed all three cistrons.
摘要
gcaD、prs和ctc基因被证明是以一个三顺反子操纵子的形式组织起来的。prs基因的转录（以磷酸核糖焦磷酸合成酶活性来衡量）以及ctc基因的转录（以ctc-lacZ蛋白融合体所指定的β-半乳糖苷酶活性来衡量）都依赖于gcaD基因前面的启动子。以gcaD-prs-ctc所指定的mRNA为模板制备的cDNA分子分析显示出一个包含所有三个顺反子的RNA转录本。
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