Maul B, Völker U, Riethdorf S, Engelmann S, Hecker M
Institut f{ur Mikrobiologie und Molekularbiologie, Universität Greifswald, Germany.
Mol Gen Genet. 1995 Jul 22;248(1):114-20. doi: 10.1007/BF02456620.
The expression of the gsiB gene of Bacillus subtilis in response to a wide variety of stress conditions was analysed, and the results provide evidence that gsiB is subject to a sigma B-dependent regulation. Primer extension experiments established identical start points for gsiB transcription during growth and after the induction by heat shock, salt or ethanol stress, and glucose limitation. The sequence upstream of the transcription start point shows great similarity to the sequences of sigma B-dependent promoters of B. subtilis. sigma B was absolutely required for the increase in gsiB mRNA level and in the synthesis rate of GsiB in response to various stimuli. Measurements of the ATP pool indicated that changes in the level of ATP might be one of the signals that regulate the activity of sigma B in B. subtilis.
分析了枯草芽孢杆菌gsiB基因在多种应激条件下的表达情况，结果表明gsiB受σB依赖性调控。引物延伸实验确定了在生长期间以及热休克、盐或乙醇应激和葡萄糖限制诱导后gsiB转录的相同起始点。转录起始点上游的序列与枯草芽孢杆菌σB依赖性启动子的序列高度相似。响应各种刺激时，σB对于gsiB mRNA水平的增加以及GsiB合成速率的增加是绝对必需的。ATP池的测量表明，ATP水平的变化可能是调节枯草芽孢杆菌中σB活性的信号之一。
