Brandt R, Léger J, Lee G
Institute of Neurobiology, University of Heidelberg, Germany.
J Cell Biol. 1995 Dec;131(5):1327-40. doi: 10.1083/jcb.131.5.1327.
The neuronal microtubule-associated protein tau is required for the development of cell polarity in cultured neurons. Using PC12 cells that stably express tau and tau amino-terminal fragments, we report that tau interacts with the neural plasma membrane through its amino-terminal projection domain. In differentiated PC12 transfectants, tau is found in growth cone-like structures in a nonmicrotubule-dependent manner. In hippocampal neurons, tau is differentially extracted by detergent and enriched in the growth cone and the distal axon when membrane is left intact. In PC12 transfectants, overexpression of tau's amino-terminal fragment, but not of full-length tau, suppresses NGF-induced process formation. Our data suggest that tau's amino-terminal projection domain has an important role in neuritic development and establishes tau as a mediator of microtubule-plasma membrane interactions.
神经元微管相关蛋白tau是培养神经元中细胞极性发育所必需的。利用稳定表达tau和tau氨基末端片段的PC12细胞，我们报告tau通过其氨基末端突出结构域与神经质膜相互作用。在分化的PC12转染细胞中，tau以非微管依赖的方式存在于生长锥样结构中。在海马神经元中，去污剂可差异提取tau，当膜保持完整时，tau在生长锥和轴突远端富集。在PC12转染细胞中，tau氨基末端片段的过表达而非全长tau的过表达抑制了NGF诱导的突起形成。我们的数据表明，tau的氨基末端突出结构域在神经突发育中具有重要作用，并确立了tau作为微管-质膜相互作用的介质。
