使用随机扩增多态性DNA分析对三带棘口吸虫和卡氏棘口吸虫进行比较。
A comparison of Echinostoma trivolvis and E. caproni using random amplified polymorphic DNA analysis.
作者信息
Fujino T, Takahashi Y, Fried B
机构信息
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
J Helminthol. 1995 Sep;69(3):263-4. doi: 10.1017/s0022149x00014243.
PMID:8522771
Abstract
The random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique was applied to two closely-related echinostome species, Echinostoma trivolvis and E. caproni, demonstrate interspecific polymorphisms of genomic DNA. Band patterns generated using five individual primers showed that these two echinostomes were genetically distinct, although they share genomic DNA to some extent.
摘要
随机扩增多态性DNA聚合酶链反应（RAPD-PCR）技术被应用于两种亲缘关系密切的棘口吸虫物种，即三歧棘口吸虫和卡氏棘口吸虫，以证明基因组DNA的种间多态性。使用五种单独引物产生的条带模式表明，这两种棘口吸虫在遗传上是不同的，尽管它们在一定程度上共享基因组DNA。
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