Petrie J L, Burg E F, Cain G D
Department of Biological Sciences, University of Iowa, Iowa City 52242, USA.
J Parasitol. 1996 Apr;82(2):360-2.
Genomic DNA from Echinostoma caproni and Echinostoma paraensei was amplified by the polymerase chain reaction using primers (5'-TCGTAGCCAA and 5'-TCACGATGCA), originally found to differentiate species and strain of Schistosoma. The 2 putative species of Echinostoma produced distinct banding patterns clearly distinguishable from one another, thereby suggesting that RAPD (random amplification of polymorphic DNA) analysis may be useful for the identification of echinostome strains and species previously misunderstood or undescribed, and that primers developed for species within a given genus, e.g., Schistosoma, may have broader application in identifying other trematodes.
使用最初发现可区分血吸虫物种和品系的引物（5'-TCGTAGCCAA和5'-TCACGATGCA），通过聚合酶链反应扩增卡氏棘口吸虫和副睾棘口吸虫的基因组DNA。这两种推定的棘口吸虫物种产生了明显不同的条带模式，彼此易于区分，从而表明随机扩增多态性DNA（RAPD）分析可能有助于鉴定之前被误解或未描述的棘口吸虫品系和物种，并且为特定属（如血吸虫属）内的物种开发的引物可能在鉴定其他吸虫方面具有更广泛的应用。
