Tamura Y, Ohya K
Department of Dental Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1995 Sep;62(3):404-9. doi: 10.5357/koubyou.62.404.
It has been recognized that metallothionein (MT) is a low molecular-weight protein and can be induced by heavy metals in several organs. Most studies on MT focus on the liver and kidney, and little is known about MT in the bone marrow. In the present study, the characterization of the MT-like protein in bone marrow of rabbits was investigated after the injection of CdCl2. A dose of 8 x 10(-5) mol/kg CdCl2 was injected subcutaneously into Japanese White Rabbits (male, 3.5 kg) for five days. Twenty-four hours after the last CdCl2 injection, the bone marrow, liver and kidney were removed immediately and frozen. The fractions of metal-binding protein were obtained by gel filtration (Sephadex G-75) of cytosol from the tissues of Cd-treated rabbit. They were heat-stable and low molecular-weight (under 10 kDa) proteins. They had high absorption at 250 nm. These observations suggest that the injection of CdCl2 induced MT-like protein in bone marrow.
人们已经认识到金属硫蛋白（MT）是一种低分子量蛋白质，可被几种器官中的重金属诱导产生。大多数关于MT的研究都集中在肝脏和肾脏，而对骨髓中的MT了解甚少。在本研究中，注射氯化镉后，对兔骨髓中类MT蛋白的特性进行了研究。将8×10⁻⁵mol/kg的氯化镉皮下注射到日本白兔（雄性，3.5kg）体内，持续五天。最后一次注射氯化镉24小时后，立即取出骨髓、肝脏和肾脏并冷冻。通过对镉处理兔组织的细胞溶质进行凝胶过滤（Sephadex G - 75）获得金属结合蛋白组分。它们是热稳定的低分子量（低于10kDa）蛋白质。它们在250nm处有高吸收。这些观察结果表明，注射氯化镉可诱导兔骨髓中产生类MT蛋白。
