血清素通过多巴胺摄取位点增强体内纹状体多巴胺外流。

Serotonin enhances striatal dopamine outflow in vivo through dopamine uptake sites.

作者信息

De Deurwaerdère P, Bonhomme N, Lucas G, Le Moal M, Spampinato U

机构信息

Université de Bordeaux II, INSERM U. 259, Bordeaux, France.

出版信息

J Neurochem. 1996 Jan;66(1):210-5. doi: 10.1046/j.1471-4159.1996.66010210.x.

DOI:10.1046/j.1471-4159.1996.66010210.x

PMID:8522956

Abstract

Serotonin (5-HT) administered at 1, 3, and 10 microM into the striatum of halothane-anesthetized rats by in vivo microdialysis increased extracellular dopamine (DA) in a concentration-dependent manner (approximately 65, 190, and 440%, respectively). These effects were reduced by 50% in the presence of 1 microM tetrodotoxin (TTX) or in the absence of Ca2+ ions. The DA uptake blocker nomifensine (0.1 microM) significantly lowered (by 50%) the enhancement of DA outflow induced by 3 microM 5-HT. Nomifensine (1 microM) coperfused with 1 microM TTX abolished the 1 and 3 microM 5-HT-induced DA outflow, whereas the effect of 10 microM 5-HT was significantly reduced by 1 (-55%) and 10 micro M (-70%) nomifensine. These data demonstrate that, in vivo, striatal DA uptake sites are partially involved in the DA-releasing action of 5-HT.

摘要

通过体内微透析向氟烷麻醉大鼠的纹状体中分别注射1、3和10微摩尔的5-羟色胺（5-HT），细胞外多巴胺（DA）以浓度依赖的方式增加（分别约为65%、190%和440%）。在存在1微摩尔河豚毒素（TTX）或不存在Ca2+离子的情况下，这些作用降低了50%。多巴胺摄取阻断剂诺米芬辛（0.1微摩尔）显著降低（50%）了由3微摩尔5-HT诱导的DA流出增强。诺米芬辛（1微摩尔）与1微摩尔TTX共同灌注消除了1和3微摩尔5-HT诱导的DA流出，而10微摩尔5-HT的作用在1微摩尔（-55%）和10微摩尔（-70%）诺米芬辛作用下显著降低。这些数据表明，在体内，纹状体DA摄取位点部分参与了5-HT的DA释放作用。