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本文引用的文献

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Overexpression, purification, and late transcription factor activity of the 17-kilodalton protein encoded by the vaccinia virus A1L gene.痘苗病毒A1L基因编码的17千道尔顿蛋白的过表达、纯化及晚期转录因子活性
J Virol. 1993 Oct;67(10):5740-8. doi: 10.1128/JVI.67.10.5740-5748.1993.
2
Purification of the late transcription system of vaccinia virus: identification of a novel transcription factor.痘苗病毒晚期转录系统的纯化：一种新型转录因子的鉴定。
J Virol. 1993 Dec;67(12):7264-70. doi: 10.1128/JVI.67.12.7264-7270.1993.
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Modification of the cascade model for regulation of vaccinia virus gene expression: purification of a prereplicative, late-stage-specific transcription factor.痘苗病毒基因表达调控级联模型的修正：一种复制前晚期特异性转录因子的纯化
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The H4 subunit of vaccinia virus RNA polymerase is not required for transcription initiation at a viral late promoter.痘苗病毒RNA聚合酶的H4亚基对于病毒晚期启动子处的转录起始并非必需。
J Virol. 1995 Apr;69(4):2602-4. doi: 10.1128/JVI.69.4.2602-2604.1995.
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Energy requirement for specific transcription initiation by the human RNA polymerase II system.人RNA聚合酶II系统特定转录起始的能量需求。
J Biol Chem. 1984 Apr 25;259(8):5321-6.
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Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits.痘病毒、原核生物和真核生物RNA聚合酶之间的同源性：编码147-kDa和22-kDa亚基的痘苗病毒基因的核苷酸序列及转录分析
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Vaccinia virus late transcripts generated in vitro have a poly(A) head.在体外产生的痘苗病毒晚期转录本有一个聚腺苷酸尾。
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Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.基于合成噬菌体T7 RNA聚合酶的重组痘苗病毒的真核瞬时表达系统。
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DNA-dependent RNA polymerase subunits encoded within the vaccinia virus genome.痘苗病毒基因组内编码的依赖DNA的RNA聚合酶亚基。
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Identification of factors specific for transcription of the late class of vaccinia virus genes.痘苗病毒晚期基因转录特异性因子的鉴定
J Virol. 1989 Oct;63(10):4224-33. doi: 10.1128/JVI.63.10.4224-4233.1989.

痘苗病毒晚期启动子的体外转录需要A2L中间基因产物。

The A2L intermediate gene product is required for in vitro transcription from a vaccinia virus late promoter.

作者信息

Hubbs A E, Wright C F

机构信息

Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000, USA.

出版信息

J Virol. 1996 Jan;70(1):327-31. doi: 10.1128/JVI.70.1.327-331.1996.

DOI:10.1128/JVI.70.1.327-331.1996
PMID:8523544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC189821/
Abstract

Previously, the in vitro late transcription system of vaccinia virus was resolved into four components: the 17- and 30-kDa products of the A1L and G8R intermediate genes, respectively, the viral DNA-dependent RNA polymerase, and an unmapped factor sedimenting at 32 to 38 kDa. Another protein, the 26-kDa product of the A2L open reading frame was predicted to be a late transcription factor on the basis of a transient-expression assay but was not recognized as being necessary for transcriptional activity in vitro. We now report that both the unmapped factor and the 26-kDa protein are required for transcription from a vaccinia virus late promoter in vitro. Since the 26-kDa protein has now been shown to be a trans-activator of late transcription and it is the product of a known gene, we suggest that it be designated VLTF-3.

摘要

以前，痘苗病毒的体外晚期转录系统可分解为四个组分：分别是A1L和G8R中间基因的17 kDa和30 kDa产物、病毒DNA依赖性RNA聚合酶以及一种沉降系数为32至38 kDa的未定位因子。另一种蛋白质，即A2L开放阅读框的26 kDa产物，基于瞬时表达试验被预测为晚期转录因子，但在体外转录活性中未被认为是必需的。我们现在报告，未定位因子和26 kDa蛋白质在体外从痘苗病毒晚期启动子转录时都是必需的。由于现在已证明26 kDa蛋白质是晚期转录的反式激活因子，并且它是一个已知基因的产物，我们建议将其命名为VLTF-3。