痘苗病毒晚期启动子的体外转录需要A2L中间基因产物。
The A2L intermediate gene product is required for in vitro transcription from a vaccinia virus late promoter.
作者信息
Hubbs A E, Wright C F
机构信息
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000, USA.
出版信息
J Virol. 1996 Jan;70(1):327-31. doi: 10.1128/JVI.70.1.327-331.1996.
Abstract
Previously, the in vitro late transcription system of vaccinia virus was resolved into four components: the 17- and 30-kDa products of the A1L and G8R intermediate genes, respectively, the viral DNA-dependent RNA polymerase, and an unmapped factor sedimenting at 32 to 38 kDa. Another protein, the 26-kDa product of the A2L open reading frame was predicted to be a late transcription factor on the basis of a transient-expression assay but was not recognized as being necessary for transcriptional activity in vitro. We now report that both the unmapped factor and the 26-kDa protein are required for transcription from a vaccinia virus late promoter in vitro. Since the 26-kDa protein has now been shown to be a trans-activator of late transcription and it is the product of a known gene, we suggest that it be designated VLTF-3.
摘要
以前，痘苗病毒的体外晚期转录系统可分解为四个组分：分别是A1L和G8R中间基因的17 kDa和30 kDa产物、病毒DNA依赖性RNA聚合酶以及一种沉降系数为32至38 kDa的未定位因子。另一种蛋白质，即A2L开放阅读框的26 kDa产物，基于瞬时表达试验被预测为晚期转录因子，但在体外转录活性中未被认为是必需的。我们现在报告，未定位因子和26 kDa蛋白质在体外从痘苗病毒晚期启动子转录时都是必需的。由于现在已证明26 kDa蛋白质是晚期转录的反式激活因子，并且它是一个已知基因的产物，我们建议将其命名为VLTF-3。
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