Saeki T, Salomon D S, Johnson G R, Gullick W J, Mandai K, Yamagami K, Moriwaki S, Tanada M, Takashima S, Tahara E
Department of Clinical Research, Pathology and Surgery, National Shikoku Cancer Center Hospital, Matsuyama.
Jpn J Clin Oncol. 1995 Dec;25(6):240-9.
The frequency of expression and localization of cripto-1 (CR-1), amphiregulin (AR), transforming growth factor alpha (TGF alpha), epidermal growth factor receptor (EGFR) and erbB-2 were examined by immunohistochemistry in 45 carcinomas and adjacent non-involved normal colon mucosa. Thirty (66.7%), 24 (53.3%), 23 (51.1%), 23 (51.1%) and 13 (28.9%) of the 45 carcinomas showed positive staining for CR-1, AR, TGF alpha, EGFR and erbB-2, respectively, whereas 7 (15.5%), 17 (37.7%), 15 (33.3%), 20 (44.4%) and 0 (0%) of the corresponding non-involved normal mucosa specimens were reactive. Among 13 carcinomas with lymph node involvement, 10 (76.9%), 8 (61.5%), 10 (76.9%), 8 (61.5%) and 7 (53.8%) exhibited positive staining for CR-1, AR, TGF-alpha, EGFR and erbB-2, respectively. There was a statistically significant association between the frequency of either TGF alpha (P < 0.05) or erbB-2 (P < 0.05) expression and lymph node metastasis. In addition, a significantly higher frequency of positive staining for TGF alpha was observed in Dukes' grade C carcinomas (P < 0.05). Finally, significant trends for coexpression of EGFR and either TGF alpha (P < 0.01) or AR (P < 0.05) were detected in carcinomas. These data suggest that AR and TGF alpha may play an important role in the development of colorectal carcinomas through an autocrine mechanism involving EGFR, and demonstrate that TGF alpha and erbB-2 may be more reliable indicators of metastasis or prognosis than CR-1, AR or EGFR in human colon cancers.
采用免疫组织化学方法检测了45例结肠癌及相邻未受累正常结肠黏膜中cripto-1（CR-1）、双调蛋白（AR）、转化生长因子α（TGFα）、表皮生长因子受体（EGFR）和erbB-2的表达频率及定位。45例结肠癌中，分别有30例（66.7%）、24例（53.3%）、23例（51.1%）、23例（51.1%）和13例（28.9%）的CR-1、AR、TGFα、EGFR和erbB-2呈阳性染色，而相应的未受累正常黏膜标本中分别有7例（15.5%）、17例（37.7%）、15例（33.3%）、20例（44.4%）和0例（0%）有反应。在13例有淋巴结转移的癌中，分别有10例（76.9%）、8例（61.5%）、10例（76.9%）、8例（61.5%）和7例（53.8%）的CR-1、AR、TGF-α、EGFR和erbB-2呈阳性染色。TGFα（P<0.05）或erbB-2（P<0.05）的表达频率与淋巴结转移之间存在统计学上的显著关联。此外，在Dukes' C级癌中观察到TGFα阳性染色频率显著更高（P<0.05）。最后，在癌中检测到EGFR与TGFα（P<0.01）或AR（P<0.05）共表达的显著趋势。这些数据表明，AR和TGFα可能通过涉及EGFR的自分泌机制在结直肠癌的发生发展中起重要作用，并表明在人类结肠癌中，TGFα和erbB-2可能比CR-1、AR或EGFR更可靠地作为转移或预后指标。
