Minamishima Y, Eizuru Y, Yoshida A, Fukunishi R
Microbiol Immunol. 1978;22(11):693-700. doi: 10.1111/j.1348-0421.1978.tb00422.x.
Murine cytomegalovirus was utilized as a model for human cytomegalovirus, which had no experimental animal, to study immunoprophylaxis of the cytomegalovirus infections. (1) Murine cytomegalovirus (MCMV) serially propagated in mouse embryonic fibroblasts had lost pathogenicity for weanling mice including neonatally thymectomized mice. (2) The cell culture-adapted MCMV was effective as a "live, attenuated virus vaccine" against challenge by virulent, mouse-passaged MCMV. (3) The immunization via intraperitoneal route protected mice from every parameter of MCMV infection. These included clinical signs, virus replication, histopathology and mortality. (4) The protective immunity was active against the virulent MCMV which was not neutralized by the rabbit anti-attenuated MCMV serum.
鼠巨细胞病毒被用作人类巨细胞病毒的模型（人类巨细胞病毒没有实验动物），以研究巨细胞病毒感染的免疫预防。（1）在小鼠胚胎成纤维细胞中连续传代的鼠巨细胞病毒（MCMV）对断奶小鼠（包括新生期胸腺切除的小鼠）已失去致病性。（2）细胞培养适应的MCMV作为“减毒活病毒疫苗”，对强毒的、经小鼠传代的MCMV攻击有效。（3）通过腹腔途径免疫可保护小鼠免受MCMV感染的各个参数影响。这些参数包括临床症状、病毒复制、组织病理学和死亡率。（4）保护性免疫对未被兔抗减毒MCMV血清中和的强毒MCMV具有活性。
