Ohashi T, Nawa Y, Minamishima Y, Owhashi M
Department of Microbiology, Miyazaki Medical College, Japan.
Arch Virol. 1988;101(3-4):237-46. doi: 10.1007/BF01311004.
The susceptibility of congenitally anemic W/WV mice to infection with murine cytomegalovirus (MCMV) was examined. W/WV mice showed a higher mortality rate and shorter survival time after MCMV infection than did their +/+ littermate mice. In addition, W/WV mice showed a lower plaque-forming unit (PFU) per 50% lethal dose (LD50) and produced higher titers of infectious virus in various organs. The mortality rate and survival time of W/WV mice which received a bone marrow graft 4 weeks before infection was completely restored to the level for +/+ mice, suggesting the importance of the cells of myeloid origin. Although natural killer (NK) activity of W/WV mice was comparable to that of +/+ mice before infection, marked reduction was observed after MCMV infection. Furthermore, OK-432 treatment failed to enhance NK activity of W/WV mice. Impaired NK response was also completely restored by bone marrow grafting 4 weeks before infection. The level of serum interferon (IFN) of infected or uninfected W/WV mice was comparable to that of +/+ mice. Therefore, impaired NK inducibility seems to be responsible, at least in part, for the high susceptibility of W/WV mice to MCMV infection.
研究了先天性贫血的W/WV小鼠对鼠巨细胞病毒（MCMV）感染的易感性。与它们的+/+同窝小鼠相比，W/WV小鼠在感染MCMV后表现出更高的死亡率和更短的存活时间。此外，W/WV小鼠每50%致死剂量（LD50）的空斑形成单位（PFU）较低，并且在各个器官中产生更高滴度的传染性病毒。在感染前4周接受骨髓移植的W/WV小鼠的死亡率和存活时间完全恢复到+/+小鼠的水平，这表明髓系来源细胞的重要性。虽然W/WV小鼠在感染前的自然杀伤（NK）活性与+/+小鼠相当，但在MCMV感染后观察到明显降低。此外，OK-432治疗未能增强W/WV小鼠的NK活性。感染前4周进行骨髓移植也能完全恢复受损的NK反应。感染或未感染的W/WV小鼠的血清干扰素（IFN）水平与+/+小鼠相当。因此，NK诱导受损似乎至少部分地导致了W/WV小鼠对MCMV感染的高易感性。
