Shin T, Nahm I, Maeyama T, Miyazaki J, Matsuo H, Yu Y
Department of Otolaryngology, Saga Medical School, Japan.
Laryngoscope. 1995 Dec;105(12 Pt 1):1315-21. doi: 10.1288/00005537-199512000-00010.
The distribution of laryngeal taste buds (TBs) and their neutral components in the cat were investigated by immunohistochemistry and electron microscopy. The antisera used in this study were against cytokeratin, protein gene product 9.5 (PGP9.5), neuron-specific enolase (NSE), S-100 protein, calbindin D, calcitonin gene-related peptide (CGRP), and substance P (SP). Taste bud cells were specifically immunoreactive to the antibodies of human cytokeratin subtypes 8 and 18 (CAM5.2). On observation with CAM5.2, TBs were seen distributed on the laryngeal surface of the epiglottis and spread caudally along the aryepiglottic folds, reaching peak density at the laryngeal side of the arytenoid tubercle. The PGP9.5 and NSE immunoreactivities were recognized in TB cells and nerve fibers, both within the TBs and in the subepithelial connective tissue. S-100 protein immunoreactivities were not found in any of the cells in the TBs but were found exclusively in the subepithelial neural elements. The calbindin-D, CGRP, and SP immunoreactivities were confined to a part of the neural elements that was very thin. Taste pores, taste villi, neuronal varicosity, and synapselike structures were observed by scanning and transmission electron microscopic study. From these results it is considered that the TBs act as a chemical receptor.
通过免疫组织化学和电子显微镜研究了猫喉味蕾（TBs）及其神经成分的分布。本研究中使用的抗血清针对细胞角蛋白、蛋白基因产物9.5（PGP9.5）、神经元特异性烯醇化酶（NSE）、S-100蛋白、钙结合蛋白D、降钙素基因相关肽（CGRP）和P物质（SP）。味蕾细胞对人细胞角蛋白亚型8和18（CAM5.2）的抗体具有特异性免疫反应。用CAM5.2观察时，可见味蕾分布在会厌的喉面，并沿杓会厌襞向尾侧延伸，在杓状软骨结节的喉侧达到密度峰值。在味蕾内和上皮下结缔组织中的味蕾细胞和神经纤维中均检测到PGP9.5和NSE免疫反应性。在味蕾的任何细胞中均未发现S-100蛋白免疫反应性，仅在上皮下神经成分中发现。钙结合蛋白D、CGRP和SP免疫反应性局限于非常细的一部分神经成分。通过扫描和透射电子显微镜研究观察到了味孔、味绒毛、神经元膨体和突触样结构。从这些结果可以认为，味蕾起到化学感受器的作用。
