Yoshie S, Teraki Y, Iwanaga T, Fujita T
Department of Oral Anatomy, Nippon Dental University, Niigata, Japan.
Arch Histol Cytol. 1989;52 Suppl:389-96. doi: 10.1679/aohc.52.suppl_389.
The taste buds and associated nerves in the guinea pig, rat, cat, and mouse were investigated by immunocytochemistry and formaldehyde-induced fluorescence histochemistry. The antisera used were against spot 35 protein, neuron-specific enolase (NSE), neurofilament protein (NFP), and substance P. The spot 35 protein immunoreactivity was confined to taste bud cells in the guinea pig and rat; the immunoreactive cells, slender in shape, comprised half the number of the total taste bud cells in the guinea pig but were fewer in the rat. For NSE, on the other hand, taste bud cells as well as neural elements localized in both the taste bud and the subepithelial connective tissue were immunoreactive in all the species investigated. Furthermore, all of the spot 35 protein-immunoreactive cells proved to be NSE-immunoreactive in the guinea pig and rat. For NFP, neither the bud cells nor the nerves in the taste bud were reactive, whereas a part of nerves in the connective tissue was immunostained in all the species. The antiserum against substance P exclusively detected some parts of nerves in and out of the taste buds in the cat, rat, and mouse. The aminergic innervation was rather meager and appeared in the nerve fibers localized in the taste buds and connective tissue of the cat and mouse.
通过免疫细胞化学和甲醛诱导荧光组织化学法对豚鼠、大鼠、猫和小鼠的味蕾及相关神经进行了研究。所用抗血清针对斑点35蛋白、神经元特异性烯醇化酶（NSE）、神经丝蛋白（NFP）和P物质。斑点35蛋白免疫反应性仅限于豚鼠和大鼠的味蕾细胞；这些免疫反应性细胞形状细长，在豚鼠中占味蕾细胞总数的一半，但在大鼠中较少。另一方面，对于NSE，在所研究的所有物种中，味蕾细胞以及位于味蕾和上皮下结缔组织中的神经成分均具有免疫反应性。此外，在豚鼠和大鼠中，所有斑点35蛋白免疫反应性细胞均被证明为NSE免疫反应性。对于NFP，味蕾细胞和味蕾中的神经均无反应，而在所有物种中，结缔组织中的部分神经被免疫染色。抗P物质抗血清仅在猫、大鼠和小鼠的味蕾内外检测到部分神经。胺能神经支配相当稀少，出现在猫和小鼠味蕾及结缔组织中的神经纤维中。
