粟酒裂殖酵母的rad18基因定义了参与DNA修复的SMC超家族的一个新亚组。
The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair.
作者信息
Lehmann A R, Walicka M, Griffiths D J, Murray J M, Watts F Z, McCready S, Carr A M
机构信息
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, United Kingdom.
出版信息
Mol Cell Biol. 1995 Dec;15(12):7067-80. doi: 10.1128/MCB.15.12.7067.
Abstract
The rad18 mutant of Schizosaccharomyces pombe is very sensitive to killing by both UV and gamma radiation. We have cloned and sequenced the rad18 gene and isolated and sequenced its homolog from Saccharomyces cerevisiae, designated RHC18. The predicted Rad18 protein has all the structural properties characteristic of the SMC family of proteins, suggesting a motor function--the first implicated in DNA repair. Gene deletion shows that both rad18 and RHC18 are essential for proliferation. Genetic and biochemical analyses suggest that the product of the rad18 gene acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair. This second repair pathway involves the products of the rhp51 gene (the homolog of the RAD51 gene of S. cerevisiae) and the rad2 gene.
摘要
粟酒裂殖酵母的rad18突变体对紫外线和γ辐射杀伤极为敏感。我们已克隆并测序了rad18基因，并从酿酒酵母中分离并测序了其同源物，命名为RHC18。预测的Rad18蛋白具有SMC蛋白家族的所有结构特性，表明其具有一种运动功能——这是首次涉及DNA修复的功能。基因缺失表明rad18和RHC18对细胞增殖均至关重要。遗传和生化分析表明，rad18基因的产物在一种DNA修复途径中发挥作用，该途径用于去除紫外线诱导的DNA损伤，且不同于经典的核苷酸切除修复。这第二条修复途径涉及rhp51基因（酿酒酵母RAD51基因的同源物）和rad2基因的产物。
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