Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Nat Plants. 2023 Aug;9(8):1280-1290. doi: 10.1038/s41477-023-01461-6. Epub 2023 Jul 24.
Cryptochromes (CRYs) act as blue-light photoreceptors that regulate development and circadian rhythms in plants and animals and as navigating magnetoreceptors in migratory birds. DNA double-strand breaks (DSBs) are the most serious type of DNA damage and threaten genome stability in all organisms. Although CRYs have been shown to respond to DNA damage, whether and how they participate in DSB repair is not well understood. Here we report that Arabidopsis CRYs promote the repair of DSBs through direct interactions with ADA2b and SMC5 in a blue-light-dependent manner to enhance their interaction. Mutations in CRYs and in ADA2b lead to similar enhanced DNA damage accumulation. In response to DNA damage, CRYs are localized at DSBs, and the recruitment of SMC5 to DSBs is dependent on CRYs. These results suggest that CRY-enhanced ADA2b-SMC5 interaction promotes ADA2b-mediated recruitment of SMC5 to DSBs, leading to DSB repair.
隐花色素（CRYs）作为蓝光光受体，在动植物中调节发育和昼夜节律，并作为候鸟的导航磁受体。DNA 双链断裂（DSBs）是最严重的 DNA 损伤类型，威胁着所有生物的基因组稳定性。尽管已经表明 CRYs 对 DNA 损伤有反应，但它们是否以及如何参与 DSB 修复尚不清楚。在这里，我们报告说，拟南芥中的 CRYs 通过与 ADA2b 和 SMC5 的直接相互作用，以依赖蓝光的方式促进 DSB 的修复，从而增强它们的相互作用。CRYs 和 ADA2b 的突变导致类似的增强 DNA 损伤积累。在 DNA 损伤的情况下，CRYs 定位在 DSB 上，并且 SMC5 募集到 DSB 上依赖于 CRYs。这些结果表明，CRY 增强的 ADA2b-SMC5 相互作用促进了 ADA2b 介导的 SMC5 募集到 DSBs，从而导致 DSB 修复。
