Rylatt D, Wilson K, Kemp B E, Elms M J, Manickavasagam B, Shi W, Cox A, McArthur M J, O'Hara J, Corbett M E
Agen Biomedical Ltd, Brisbane, Australia.
Prog Clin Biol Res. 1995;392:273-84.
A rapid whole blood agglutination test has been developed for the detection of endotoxin. The test reagent consists of polymyxin B (PmB) conjugated to the Fab fragment of the anti-glycophorin antibody 1C3/86. After addition of reagent to whole blood, red cell agglutination occurs within two minutes in samples from endotoxaemic patients or with the addition of either whole Gram negative bacteria, supernatants from Gram negative bacterial cultures or purified endotoxin. In clinical samples there was a strong correlation (r = 0.83) between the strength of agglutination and the level of endotoxin measured by the Limulus amaebolysate test (LAL). The prospect of a rapid and accurate test for endotoxin may enable better clinical management of Gram negative sepsis.
已开发出一种用于检测内毒素的快速全血凝集试验。该试验试剂由与抗血型糖蛋白抗体1C3/86的Fab片段偶联的多粘菌素B（PmB）组成。将试剂加入全血后，在内毒素血症患者的样本中，或加入全革兰氏阴性菌、革兰氏阴性菌培养上清液或纯化内毒素后，两分钟内会发生红细胞凝集。在临床样本中，凝集强度与通过鲎试剂试验（LAL）测定的内毒素水平之间存在很强的相关性（r = 0.83）。一种快速准确的内毒素检测方法有望实现对革兰氏阴性菌败血症更好的临床管理。
