Pébay M, Holl A C, Simonet J M, Decaris B
Laboratoire de Génétique et Microbiologie, UA INRA 952, Université Henry Poincaré Nancy 1, Faculté des Sciences, Vandoeuvre-lès-Nancy, France.
Res Microbiol. 1995 Jun;146(5):371-83. doi: 10.1016/0923-2508(96)80283-x.
Cloning and characterization of the gor gene of the lactic acid bacterium Streptococcus thermophilus, encoding glutathione reductase, are described in this paper. This enzyme is a part of the enzymatic defences against oxidative stress in eukaryotic cells and in Gram-negative bacteria, but was never found in Gram-positive bacteria before this study. The amino acid sequence shares extensive similarities with glutathione reductases from other organisms, e.g. 62% amino acid identity with Escherichia coli protein. Northern blot analysis and glutathione reductase enzyme assays gave evidence that the gene is expressed in aerobically growing cells.
本文描述了嗜热链球菌乳酸菌gor基因的克隆与特性，该基因编码谷胱甘肽还原酶。这种酶是真核细胞和革兰氏阴性菌中抗氧化应激酶防御体系的一部分，但在本研究之前从未在革兰氏阳性菌中发现过。其氨基酸序列与其他生物的谷胱甘肽还原酶有广泛的相似性，例如与大肠杆菌蛋白的氨基酸同一性为62%。Northern印迹分析和谷胱甘肽还原酶活性测定表明该基因在需氧生长的细胞中表达。
