Cvetnić Z, Pepeljnjak S
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia.
Z Lebensm Unters Forsch. 1995 Oct;201(4):399-401. doi: 10.1007/BF01192741.
UV photography in glucose, yeast extract (GY) agar medium was tested as a simple and rapid method for the distinction of aflatoxin-positive from aflatoxin-negative strains of Aspergillus flavus and A. parasiticus. In the UV photographs aflatoxin-producing moulds were identified as grey or black colonies, whereas aflatoxin-nonproducing moulds appeared as white colonies. Of the aflatoxin-positive strains detected by the UV photographic method, 10% was confirmed by extraction of the GY agar medium and mould mycelium in chloroform, extracts which were analysed subsequently using thin-layer chromatography. Confirmation of aflatoxigenic strains was achieved by biosynthesis on liquid medium yeast extract sucrose (YES) broth.
在葡萄糖酵母提取物（GY）琼脂培养基中进行紫外线摄影，作为一种区分黄曲霉和寄生曲霉产黄曲霉毒素菌株与不产黄曲霉毒素菌株的简单快速方法进行了测试。在紫外线照片中，产黄曲霉毒素的霉菌被鉴定为灰色或黑色菌落，而不产黄曲霉毒素的霉菌则呈现为白色菌落。通过紫外线摄影法检测出的产黄曲霉毒素阳性菌株中，有10%通过用氯仿提取GY琼脂培养基和霉菌菌丝体得到证实，随后对提取物进行薄层色谱分析。通过在液体培养基酵母提取物蔗糖（YES）肉汤上进行生物合成来确认产黄曲霉毒素的菌株。
