Abarca M L, Bragulat M R, Bruguera M T, Cabañes F J
Dpto. Patología y Producciones Animales (Microbiología), Facultad de Veterinaria, Bellaterra Barcelona, Spain.
Mycopathologia. 1988 Nov;104(2):75-9. doi: 10.1007/BF00436930.
Thirty seven strains of the Aspergillus flavus group isolated from animal mixed feeds have been screened for their ability to produce aflatoxins in yeast extract and sucrose (YES), aflatoxin producing ability (APA), and coconut agar medium (CAM) media. The concentration and detection of the aflatoxins by different methods is compared. Five known aflatoxin-positive and one aflatoxin-negative strains have been used as controls. Only 5 out of the 37 strains (13.5%) were aflatoxin-producers in YES medium. Of these five strains and the five known aflatoxin-positive strains, only three showed blue fluorescence in APA medium and four in CAM medium. Generally, the aflatoxin concentration in CAM medium was higher than in YES and APA media. Using the 'agar-plug method' and by direct spotting of the YES broth on TLC plates, some aflatoxin-producing strains were not detected.
从动物混合饲料中分离出的37株黄曲霉群菌株，已针对其在酵母提取物和蔗糖（YES）、黄曲霉毒素产生能力（APA）以及椰子琼脂培养基（CAM）中产生黄曲霉毒素的能力进行了筛选。比较了通过不同方法对黄曲霉毒素的浓度检测。使用了5株已知的黄曲霉毒素阳性菌株和1株黄曲霉毒素阴性菌株作为对照。在YES培养基中，37株菌株中只有5株（13.5%）是黄曲霉毒素产生菌。在这5株菌株和5株已知的黄曲霉毒素阳性菌株中，只有3株在APA培养基中显示蓝色荧光，4株在CAM培养基中显示蓝色荧光。一般来说，CAM培养基中的黄曲霉毒素浓度高于YES和APA培养基。使用“琼脂块法”并将YES肉汤直接点样在薄层色谱板上时，一些产黄曲霉毒素的菌株未被检测到。
