Romanova L V, Mishan'kin B N
Zh Mikrobiol Epidemiol Immunobiol. 1995 Sep-Oct(5):77-80.
Primers for the detection of F.tularensis in the polymerase chain reaction (PCR) have been obtained. The method of PCR permits the identification of up to 10 F.tularensis cells in the analysis of pure and mixed cultures, as well as suspensions prepared from the organs of laboratory animals. The possibility of the simultaneous analysis of several samples makes this system for the detection of F.tularensis useful for epidemiological investigations.
已获得用于在聚合酶链反应(PCR)中检测土拉弗朗西斯菌的引物。PCR方法能够在对纯培养物、混合培养物以及从实验动物器官制备的悬液进行分析时鉴定出多达10个土拉弗朗西斯菌细胞。同时分析多个样本的可能性使得这种检测土拉弗朗西斯菌的系统对流行病学调查很有用。