通过聚合酶链反应检测血液中的土拉弗朗西斯菌。
Detection of Francisella tularensis in blood by polymerase chain reaction.
作者信息
Long G W, Oprandy J J, Narayanan R B, Fortier A H, Porter K R, Nacy C A
机构信息
Center for Immunization Research, Johns Hopkins University, Baltimore, Maryland 21205.
出版信息
J Clin Microbiol. 1993 Jan;31(1):152-4. doi: 10.1128/jcm.31.1.152-154.1993.
We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.
我们开发了一种基于聚合酶链反应的土拉弗朗西斯菌检测方法，并通过使用加标血样和实验感染小鼠对其进行了评估。该检测方法能检测到A型和B型土拉弗朗西斯菌，其检测水平相当于每微升加标血样中有一个菌落形成单位（CFU）。对感染了活疫苗株的小鼠血液进行有限稀释后，基于聚合酶链反应的检测结果与血培养结果非常吻合。
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