Franke H
Institute of Pharmacology and Toxicology, University of Leipzig, Germany.
Acta Histochem. 1995 Jul;97(3):263-71. doi: 10.1016/S0065-1281(11)80187-X.
The influence of long term application of 5% (v/v) ethanol over a period of 36 weeks and 10% (v/v) ethanol over a period of 4,12 and 36 weeks to Wistar rats was investigated. The qualitative alterations of astrocytes and quantitative changes of glial fibrillary acidic protein-immunoreactivity (GFAP-IR) in selected regions of the dorsal hippocampus were examined, using anti-GFAP and the avidin-biotin immunoperoxidase technique. After prolonged (36 weeks) consumption of 5% (v/v) ethanol insignificant alterations were observed. The administration of 10% (v/v) ethanol over 4 and 12 weeks led to an increase of the total GFAP-IR in the examined brain regions. Hypertrophy of the cell bodies and cytoplasmic processes were seen. After 36 weeks decreased total GFAP-IR was measured in all examined brain regions. Cell bodies and fibrillary processes stained less heavily, the number and length of the fibrillary processes decreased and the number of astrocytes in certain hippocampal regions (e.g. in stratum moleculare of the dentate gyrus) appeared reduced. The results show that exposition to ethanol led to the appearance of different astrocytic phenotypes, depending on the concentration and duration of ethanol application, on the age of animals and on hippocampal regions. It is suggested that GFAP can be used as a specific marker for ethanol-induced alterations of astrocytes.
研究了对Wistar大鼠长期应用36周5%（体积/体积）乙醇以及分别应用4周、12周和36周10%（体积/体积）乙醇的影响。使用抗胶质纤维酸性蛋白（GFAP）和抗生物素蛋白-生物素免疫过氧化物酶技术，检测了背侧海马选定区域星形胶质细胞的定性改变和胶质纤维酸性蛋白免疫反应性（GFAP-IR）的定量变化。长期（36周）饮用5%（体积/体积）乙醇后，未观察到明显改变。应用10%（体积/体积）乙醇4周和12周导致所检测脑区的总GFAP-IR增加。可见细胞体和细胞质突起肥大。36周后，所有检测脑区的总GFAP-IR均下降。细胞体和纤维状突起的染色变浅，纤维状突起的数量和长度减少，某些海马区域（如齿状回分子层）的星形胶质细胞数量似乎减少。结果表明，根据乙醇的浓度和应用持续时间、动物年龄以及海马区域的不同，接触乙醇会导致不同星形胶质细胞表型的出现。提示GFAP可作为乙醇诱导星形胶质细胞改变的特异性标志物。
