Harwig S S, Eisenhauer P B, Chen N P, Lehrer R I
Department of Medicine, UCLA-Center for the Health Sciences 90024-1678, USA.
Adv Exp Med Biol. 1995;371A:251-5. doi: 10.1007/978-1-4615-1941-6_53.
We purified three peptides ("cryptdins") from the small intestines of mice, established their primary amino acid sequences and examined their antimicrobial activity. Their primary sequences revealed approximately 50% identity to a group of antimicrobial defensins that we had previously isolated from the granules of rat neutrophils. In addition to their ability to kill Gram-positive (L. monocytogenes) and Gram-negative bacteria (E. coli and S. typhimurium) in vitro, the peptides were much more active against an avirulent (phoP) S. typhimurium strain than against its isogenic, mouse-virulent progenitor. Overall, these data suggest that endogenous antimicrobial peptides produced by Paneth cells may protect small intestinal crypts, which are critical sites of epithelial cell renewal, from invasion by autochthonous flora or by perorally acquired potential pathogens, such as Listeria and Salmonella.
我们从小鼠小肠中纯化出三种肽（“隐窝素”），确定了它们的一级氨基酸序列，并检测了它们的抗菌活性。它们的一级序列显示与我们之前从大鼠中性粒细胞颗粒中分离出的一组抗菌防御素约有50%的同源性。除了在体外能够杀死革兰氏阳性菌（单核细胞增生李斯特菌）和革兰氏阴性菌（大肠杆菌和鼠伤寒沙门氏菌）外，这些肽对无毒力（phoP）的鼠伤寒沙门氏菌菌株的活性比对其同基因的、对小鼠有毒力的亲本菌株的活性要强得多。总体而言，这些数据表明潘氏细胞产生的内源性抗菌肽可能保护小肠隐窝免受自身菌群或经口获得的潜在病原体（如李斯特菌和沙门氏菌）的侵袭，而小肠隐窝是上皮细胞更新的关键部位。
