Fideler B M, Vangsness C T, Lu B, Orlando C, Moore T
Department of Orthopaedic Surgery, University of Southern California School of Medicine, Los Angeles 90033, USA.
Am J Sports Med. 1995 Sep-Oct;23(5):643-6. doi: 10.1177/036354659502300521.
Sixty 10-mm bone-patellar tendon-bone allografts from young human donors were placed into four test groups, a control fresh-frozen group and three fresh-frozen irradiated groups. The irradiated groups were exposed to 2.0, 3.0, or 4.0 Mrad of gamma irradiation. The specimens were tested to tensile failure. The initial biomechanical strength of fresh-frozen allografts was reduced up to 15% when compared with fresh-frozen controls after 2.0 Mrad of irradiation. Maximum force, strain energy, modulus, and maximum stress demonstrated a statistically significant reduction after 2.0 Mrad of irradiation (P < 0.01). Stiffness, elongation, and strain were reduced but not with statistical significance. A 10% to 24% and 19% to 46% reduction in all biomechanical properties were found after 3.0 (P < 0.005) and 4.0 (P < 0.0005) Mrad of irradiation, respectively. After irradiation with a 4.0 Mrad dose, the ultimate load was below that of reported values for the human anterior cruciate ligament. It is clinically important to observe and document changes in human ligaments that result from currently used doses of gamma irradiation. The results from this study provide important information regarding the initial biomechanical properties of fresh-frozen human bone-patellar tendon-bone allografts after bacterial sterilization with gamma irradiation. The current accepted dose for sterilization is between 1.5 and 2.5 Mrad. There appeared to be a dose-dependent effect of irradiation on all the biomechanical parameters studied. Four of seven parameters were found to be reduced after 2.0 Mrad of irradiation. Reductions were found in all parameters after 3.0 and 4.0 Mrad of irradiation.
将来自年轻人类供体的60个10毫米骨 - 髌腱 - 骨同种异体移植物分为四个测试组，一个对照新鲜冷冻组和三个新鲜冷冻辐照组。辐照组接受2.0、3.0或4.0兆拉德的伽马射线辐照。对标本进行拉伸破坏测试。与新鲜冷冻对照组相比，2.0兆拉德辐照后新鲜冷冻同种异体移植物的初始生物力学强度降低了15%。2.0兆拉德辐照后，最大力、应变能、模量和最大应力均呈现出统计学上的显著降低（P < 0.01）。刚度、伸长率和应变有所降低，但无统计学意义。3.0兆拉德（P < 0.005）和4.0兆拉德（P < 0.0005）辐照后，所有生物力学性能分别降低了10%至24%和19%至46%。4.0兆拉德剂量辐照后，极限负荷低于人类前交叉韧带的报告值。观察和记录当前使用的伽马射线辐照剂量导致的人类韧带变化在临床上具有重要意义。本研究结果提供了关于新鲜冷冻人骨 - 髌腱 - 骨同种异体移植物经伽马射线辐照进行细菌灭菌后的初始生物力学性能的重要信息。目前公认的灭菌剂量在1.5至2.5兆拉德之间。辐照对所有研究的生物力学参数似乎存在剂量依赖性效应。2.0兆拉德辐照后，七个参数中有四个降低。3.0和4.0兆拉德辐照后，所有参数均降低。
