Eras Volker, Graffunder Josefine, Ahmed Norus, Brune Jan C
German Institute for Cell and Tissue Replacement (DIZG, gemeinnützige GmbH), Haus 42, Köpenicker Str. 325, 12555, Berlin, Germany.
J Exp Orthop. 2021 Mar 5;8(1):18. doi: 10.1186/s40634-021-00336-z.
Meniscus allograft transplantation (MAT) is a possible treatment for patients suffering with pain after meniscectomy. Here, peracetic acid (PAA) sterilised meniscus transplants were investigated on whether they would provide an adequate alternative to fresh-frozen transplants in their viscoelastic and mechanical properties.
In this analysis, 31 menisci donors (26 male and 5 female) were included. The average donor age was 49.87 years, ranging from 32 to 65 years. Menisci of matched pairs of knees underwent chemical sterilisation while counterparts were left fresh-frozen. Stiffness and load to failure were determined via suture retention. Further menisci were analysed while attached to the tibial bone block using a novel test device to mimic physiological load distribution. Meniscus relaxation, stiffness and failure loads were determined. Histology and biphasic properties of the menisci were examined and results were analysed using paired t-tests.
A novel custom built test device allowed the application of physiological loads for suture retention testing and revealed no significant differences between PAA sterilised (14.85 ± 4.46 N/mm, 50.49 ± 17.01 N) and fresh-frozen (18.26 ± 4.46 N/mm, 59.49 ± 21.07 N) regarding stiffness and failure load, respectively. Furthermore, initial 200 N loading showed significantly higher strain in sterilised menisci (18.87 ± 1.56) compared to fresh frozen (13.81 ± 1.04). Load relaxation experiments demonstrated significantly lower relaxation for sterilised menisci (77.71 ± 1.62) compared to fresh-frozen (89.11 ± 1.00, p-value < 0.0001).
Peracetic acid sterilised human menisci performed equally to fresh-frozen counterparts in a suture retention test and in physiological failure testing providing an adequate alternative. However, meniscus relaxation, biphasic properties and strain were shown to be significantly different between the groups. A common problem of MAT is graft extrusion or shrinkage, therefore the parameters measured here should be considered and may influence meniscus extrusion after transplantation.
n/a (experimental study).
同种异体半月板移植（MAT）是半月板切除术后疼痛患者的一种可能治疗方法。在此，研究了过氧乙酸（PAA）消毒的半月板移植体在粘弹性和力学性能方面是否能提供与新鲜冷冻移植体相当的替代物。
本分析纳入了31名半月板供体（26名男性和5名女性）。供体平均年龄为49.87岁，范围为32至65岁。配对膝关节的半月板进行化学消毒，而对应物则新鲜冷冻保存。通过缝线保留试验测定刚度和破坏载荷。使用一种新型测试装置对附着在胫骨骨块上的半月板进行进一步分析，以模拟生理载荷分布。测定半月板的松弛度、刚度和破坏载荷。检查半月板的组织学和双相特性，并使用配对t检验分析结果。
一种新型定制测试装置可用于缝线保留试验中的生理载荷施加，结果显示，PAA消毒的半月板（14.85±4.46N/mm，50.49±17.01N）和新鲜冷冻的半月板（18.26±4.46N/mm，59.49±21.07N）在刚度和破坏载荷方面无显著差异。此外，初始200N加载时，消毒半月板（18.87±1.56）的应变显著高于新鲜冷冻半月板（13.81±1.04）。载荷松弛实验表明，消毒半月板（77.71±1.62）的松弛度显著低于新鲜冷冻半月板（89.11±1.00，p值<0.0001）。
在缝线保留试验和生理破坏试验中，过氧乙酸消毒的人半月板与新鲜冷冻的半月板表现相当，可提供充分的替代物。然而，两组之间的半月板松弛度、双相特性和应变存在显著差异。MAT的一个常见问题是移植物挤出或收缩，因此应考虑此处测量的参数，其可能会影响移植后半月板的挤出。
无（实验研究）。
