对法国一次疫情期间分离出的甲型肝炎病毒毒株进行直接测序。
Direct sequencing of hepatitis A virus strains isolated during an epidemic in France.
作者信息
Apaire-Marchais V, Robertson B H, Aubineau-Ferre V, Le Roux M G, Leveque F, Schwartzbrod L, Billaudel S
机构信息
Laboratoire de Virologie, Institut de Biologie, Nantes, France.
出版信息
Appl Environ Microbiol. 1995 Nov;61(11):3977-80. doi: 10.1128/aem.61.11.3977-3980.1995.
Abstract
Direct sequencing of PCR products was used to study the VP1 region of the hepatitis A virus (HAV) genome (position 2199 to 2356) of nine strains isolated from human stools collected during a hepatitis A epidemic (western France, 1992), three strains from environmental samples (1990, 1991, and 1992), and two HAV cell culture isolates (the French strain CF53/Lyon and strain CLF). These viruses differed from CF53/Lyon (genotype I) by between 1 and 10.3%, and results indicated the existence of two groups of strains belonging to two different subgenotypes (IA and IB). With this sequencing technique it was possible to monitor the epidemiology of HAV and study its relations.
摘要
采用聚合酶链反应（PCR）产物直接测序法，对从甲型肝炎流行期间（1992年，法国西部）采集的人类粪便中分离出的9株病毒、3株从环境样本中分离出的病毒（1990年、1991年和1992年）以及2株甲型肝炎病毒细胞培养分离株（法国CF53/Lyon株和CLF株）的甲型肝炎病毒（HAV）基因组VP1区（位置2199至2356）进行了研究。这些病毒与CF53/Lyon株（基因型I）的差异在1%至10.3%之间，结果表明存在两组属于两种不同亚基因型（IA和IB）的毒株。通过这种测序技术，可以监测甲型肝炎病毒的流行病学并研究其关系。
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