人类甲型肝炎病毒的一级结构与基因组织
Primary structure and gene organization of human hepatitis A virus.
作者信息
Najarian R, Caput D, Gee W, Potter S J, Renard A, Merryweather J, Van Nest G, Dina D
出版信息
Proc Natl Acad Sci U S A. 1985 May;82(9):2627-31. doi: 10.1073/pnas.82.9.2627.
Abstract
The RNA genome of human hepatitis A virus (HAV) was molecularly cloned. Recombinant DNA clones representing the entire HAV RNA were used to determine the primary structure of the viral genome. The length of the viral genome is 7478 nucleotides. An open reading frame starting at nucleotide 734 and terminating at nucleotide 7415 encodes a polyprotein of Mr 251,940. Comparison of the HAV nucleotide sequence with that of other picornaviruses has failed to reveal detectable areas of homology. However, a computer analysis of the putative amino acid sequence of HAV and poliovirus demonstrated the existence of short areas of homology in virion protein 3 (VP3) and throughout the carboxyl-terminal portion of the polyproteins. In addition, extensive protein structural homologies with poliovirus were detected.
摘要
人类甲型肝炎病毒（HAV）的RNA基因组被进行了分子克隆。代表整个HAV RNA的重组DNA克隆被用于确定病毒基因组的一级结构。病毒基因组长度为7478个核苷酸。一个从核苷酸734开始、到核苷酸7415终止的开放阅读框编码一个分子量为251,940的多聚蛋白。将HAV核苷酸序列与其他小核糖核酸病毒的序列进行比较，未发现可检测到的同源区域。然而，对HAV和脊髓灰质炎病毒推定氨基酸序列的计算机分析表明，在病毒体蛋白3（VP3）以及多聚蛋白的整个羧基末端部分存在短的同源区域。此外，还检测到与脊髓灰质炎病毒广泛的蛋白质结构同源性。
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