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Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp.嗜热栖热袍菌属菌株FjSS3 - B.1木聚糖酶基因的序列与表达、重组酶的特性及其对牛皮纸浆的活性

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本文引用的文献

1

Production of a cloned xylanase in Bacillus cereus and its performance in kraft pulp prebleaching.蜡样芽孢杆菌中克隆木聚糖酶的生产及其在硫酸盐浆预漂白中的性能

Can J Microbiol. 1993 Sep;39(9):853-60. doi: 10.1139/m93-127.

2

Correction of the beta-mannanase domain of the celC pseudogene from Caldocellulosiruptor saccharolyticus and activity of the gene product on kraft pulp.嗜糖栖热解纤维素菌celC假基因的β-甘露聚糖酶结构域的校正及其基因产物对硫酸盐浆的活性

Appl Environ Microbiol. 1995 Jun;61(6):2262-9. doi: 10.1128/aem.61.6.2262-2269.1995.

3

Identification of a novel cellulose-binding domain within the multidomain 120 kDa xylanase XynA of the hyperthermophilic bacterium Thermotoga maritima.嗜热栖热菌多结构域120 kDa木聚糖酶XynA中一个新型纤维素结合结构域的鉴定

Mol Microbiol. 1995 Feb;15(3):431-44. doi: 10.1111/j.1365-2958.1995.tb02257.x.

4

Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.刚果红-多糖相互作用在牛瘤胃纤维素分解菌计数与特性鉴定中的应用

Appl Environ Microbiol. 1982 Apr;43(4):777-80. doi: 10.1128/aem.43.4.777-780.1982.

5

A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。

Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.

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Use of uxu-lac fusion strains to study the regulation of the uxuAB operon in Escherichia coli K12.利用uxu-lac融合菌株研究大肠杆菌K12中uxuAB操纵子的调控。

J Gen Microbiol. 1983 Nov;129(11):3335-43. doi: 10.1099/00221287-129-11-3335.

7

Colorimetric and fluorometric carbohydrate determination with p-hydroxybenzoic acid hydrazide.用对羟基苯甲酸酰肼进行比色和荧光法碳水化合物测定。

Biochem Med. 1973 Apr;7(2):274-81. doi: 10.1016/0006-2944(73)90083-5.

8

Amino acid substitutions in structurally related proteins. A pattern recognition approach. Determination of a new and efficient scoring matrix.结构相关蛋白质中的氨基酸替换。一种模式识别方法。一种新型高效评分矩阵的确定。

J Mol Biol. 1988 Dec 20;204(4):1019-29. doi: 10.1016/0022-2836(88)90058-7.

9

Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum.嗜热栖热放线菌木聚糖酶基因（xynZ）的核苷酸序列及缺失分析

J Bacteriol. 1988 Oct;170(10):4582-8. doi: 10.1128/jb.170.10.4582-4588.1988.

10

Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region.包含大肠杆菌atpE翻译起始区域的可诱导表达载体。

Gene. 1987;52(2-3):279-83. doi: 10.1016/0378-1119(87)90054-0.

嗜热栖热袍菌属菌株FjSS3 - B.1木聚糖酶基因的序列与表达、重组酶的特性及其对牛皮纸浆的活性

Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp.

作者信息

Saul D J, Williams L C, Reeves R A, Gibbs M D, Bergquist P L

机构信息

Centre for Gene Technology, University of Auckland, New Zealand.

出版信息

Appl Environ Microbiol. 1995 Nov;61(11):4110-3. doi: 10.1128/aem.61.11.4110-4113.1995.

DOI:10.1128/aem.61.11.4110-4113.1995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC167719/

Abstract

A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.

摘要

从嗜热栖热菌属菌株FjSS3 - B.1的基因组文库中分离出一个表达木聚糖酶活性的基因。该基因序列表明它编码一种单结构域的10家族木聚糖酶。重组酶具有极高的热稳定性、在相对较宽的pH范围内具有活性，并且对辐射松硫酸盐浆具有活性。