嗜热栖热袍菌属菌株FjSS3 - B.1木聚糖酶基因的序列与表达、重组酶的特性及其对牛皮纸浆的活性
Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp.
作者信息
Saul D J, Williams L C, Reeves R A, Gibbs M D, Bergquist P L
机构信息
Centre for Gene Technology, University of Auckland, New Zealand.
出版信息
Appl Environ Microbiol. 1995 Nov;61(11):4110-3. doi: 10.1128/aem.61.11.4110-4113.1995.
Abstract
A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.
摘要
从嗜热栖热菌属菌株FjSS3 - B.1的基因组文库中分离出一个表达木聚糖酶活性的基因。该基因序列表明它编码一种单结构域的10家族木聚糖酶。重组酶具有极高的热稳定性、在相对较宽的pH范围内具有活性，并且对辐射松硫酸盐浆具有活性。
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