快速准确地鉴定大肠杆菌K-12菌株。
Rapid and accurate identification of Escherichia coli K-12 strains.
作者信息
Kuhnert P, Nicolet J, Frey J
机构信息
Institute of Veterinary Bacteriology, University of Bern, Switzerland.
出版信息
Appl Environ Microbiol. 1995 Nov;61(11):4135-9. doi: 10.1128/aem.61.11.4135-4139.1995.
Abstract
A specific PCR for the identification of K-12 strains, based on the genetic structure of the O-antigen gene cluster (rfb) of Escherichia coli K-12, is described. The assay clearly differentiates E. coli K-12-derived strains from other E. coli strains used in the laboratory or isolated from human and animal clinical specimens, from food, or from environmental samples. Moreover, lineages of K-12 strains can be distinguished with a second PCR based on the same gene cluster. The method presents a useful tool in identifying K-12 for monitoring strains which are used as biologically safe vehicles in biotechnological research, development, and production processes.
摘要
本文描述了一种基于大肠杆菌K-12 O抗原基因簇（rfb）的遗传结构来鉴定K-12菌株的特异性聚合酶链反应（PCR）。该检测方法能够清晰地区分源自大肠杆菌K-12的菌株与实验室使用的其他大肠杆菌菌株，以及从人类和动物临床标本、食品或环境样本中分离出的菌株。此外，基于同一基因簇的第二次PCR可以区分K-12菌株的谱系。该方法为鉴定K-12提供了一种有用的工具，可用于监测在生物技术研究、开发和生产过程中用作生物安全载体的菌株。
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